Multiphoton Spectral Imaging Microscopy

描述（由申请人提供）：wm Keck细胞成像中心（KCCI）是先进光学显微镜成像，特别是蛋白质-蛋白质相互作用成像的领先中心，现申请蔡司“多光子光谱成像显微镜（MSIM）”系统。KCCI是一所大学范围内的成像设施，所要求的仪器将使该大学的基础和临床科学研究人员受益，特别是8名参与联邦资助的研究人员。该仪器将被安置在完善的KCCI，位于所有参与调查人员的步行距离内。在蔡司MSIM上进行的研究的中心主题是活细胞和组织中蛋白质-蛋白质相互作用的研究，包括：er α和辅抑制蛋白之间的配体依赖性蛋白质相互作用；线粒体!创伤性轴突发病机制的破坏；Rho家族GTPases的FRET成像tau蛋白与阿尔茨海默病的关系；原肠胚形成过程中应变纤维连接蛋白网络的形态星形胶质细胞过程的动力学及其与损伤神经元的相互作用；转基因胚胎的基因调控机制；以及细胞对抗癌药物的摄取。该系统允许应用生物物理技术，如光漂白后荧光恢复（FRAP）和荧光共振能量转移（FRET），活体标本，在原位。我们对蔡司MSIM的请求是为了响应该机构研究人员在其nih资助的项目中利用这些技术进步的日益增长的需求。KCCI是使用各种光学显微镜技术研究蛋白质-蛋白质相互作用的国际知名中心，每年为来自美国和15个国家的科学家提供实践培训。目前，我们还没有多光子光谱成像系统，这对我们的研究人员以及培训其他人研究活细胞和组织中的蛋白质关联是必不可少的。总之，所要求的仪器将为弗吉尼亚大学的研究界提供过去没有的成像能力水平，并将大大加强正在进行和未来的研究。

DESCRIPTION (provided by applicant): The W.M. Keck Center for Cellular Imaging (KCCI), a leading center in advanced light microscopy imaging, particularly in protein-protein interaction imaging, is requesting a Zeiss "Multiphoton Spectral Imaging Microscopy (MSIM)" system. KCCI is a University wide imaging facility and the requested instrument will benefit the basic and clinical science investigators of this university, and in particular the 8 participating Federally funded investigators. The instrument will be housed in the well-established KCCI, located within walking distance of all the participating investigators. The central theme of the research to be performed on the requested Zeiss MSIM is the study of protein-protein interactions in live cells and tissues including: liganddependent protein interactions between ERalpha and corepressor proteins; mitochondria! disruption in traumatic axonal pathogenesis; FRET Imaging of Rho family GTPases; association of tau protein in Alzheimer's disease; the pattern of strain fibronectin networks during gastrulation; the dynamics of astroglial processes and their interactions with injured neurons; gene regulatory mechanisms in transgenic embryos; and cellular uptake of anti-cancer drugs. This system allows the application of biophysical techniques such as fluorescence recovery after photobleaching (FRAP), and fluorescence resonance energy transfer (FRET), to live specimens, in situ. Our request for the Zeiss MSIM is in response to the growing need of researchers at this institution to take advantage of these technological advances in their NIH-funded projects. KCCI is an internationally known center in the study of protein-protein interactions using various light microscopy techniques and provides hands on training annually for scientists from the United States as well as scientists from 15 countries. Currently, we do not have a multiphoton spectral imaging system, this is essential for our investigators, as well as for training others, to study the protein associations in living cells and tissues. In summary, the requested instrument will provide the research community at the University of Virginia with a level of imaging capability that has not been available in the past and will enhance ongoing and future research significantly.