Multifrequency EPR/ENDOR Spectrometer

描述（由申请人提供）： 这项共享仪器赠款提案是为了收购一个现代化的商业CW和脉冲EPR光谱仪，供旧金山弗朗西斯科湾地区EPR用户使用，目标是生物金属和自由基中心。该仪器是一台Bruker EleXsys 580，可为连续波（CW）和脉冲EPR实验提供最先进的性能。除了标准的X波段频率（9.4 GHz），我们建议为CW和脉冲操作增加Q波段（35 GHz）选项。我们还提出了连续波和脉冲电子核双共振（ENDOR）的能力。不同的谐振器组合包括低温恒温器。主要研究机构加州大学戴维斯分校慷慨的匹配承诺，使我们能够提出这些关键的选择（Q波段，ENDOR，低温）。该仪器将为NIH资助的各种团体提供各种现代先进的EPR方法，检查顺磁性金属或自由基中心，无论是天然存在于酶中还是作为自旋探针添加。

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant proposal is for the acquisition of a modern commercial CW and Pulsed EPR spectrometer for use by San Francisco Bay area EPR users targeting biological metal and radical centers. The proposed instrument, a Bruker EleXsys 580, offers state-of-the-art performance for both continuous wave (CW) and pulsed EPR experiments. In addition to the standard X-band frequency (9.4 GHz), we propose to add the Q-band (35 GHz) option for both CW and pulsed operations. We are also proposing both CW and pulsed electron nuclear double resonance (ENDOR) capabilities. Low temperature cryostats are included for the different resonator combinations. A generous matching commitment by the Principal Investigator's institution, UC-Davis, allows us to propose these crucial options (Q-band, ENDOR, cryogenics). This instrument will provide access to a variety of modern advanced EPR methods for a wide array of NIH funded groups examining paramagnetic metal or radical centers, either naturally occurring in enzymes or added as spin probes.