Lipid Core

该核心将为中心的每个项目提供基本的脂质和脂蛋白分析。这一核心已经存在了12年多，因此，核心所采用的方法是高度发达的。详细描述了每个程序所使用的技术。核心将提供：使用标准化酶测定法分析血浆脂质浓度;通过GC/MS分析脂肪酸和胆固醇酯;通过HPLC分析胆固醇和甘油三酯的血浆脂蛋白分布;使用主要脂质类别的酶测定法分析血浆和组织脂质组成;通过GLC分析主要脂质类别的脂质脂肪酸组成;通过GLC分析胆固醇和胆固醇酯浓度;通过HPLC分析白三烯和HETE。这个核心实验室所提供的技术将极大地促进每个项目的研究。

This core will provide basic lipid and lipoprotein analyses for each project in the Center. This core has been in existence for over 12 years and the methodology to be employed by the core is therefore highly developed. The techniques for each of the procedures to be used are described in detail. The core will provide: analysis of plasma lipid concentrations using standardized enzymatic assays; analysis of fatty acids and prostaglandins by GC/MS; analysis of plasma lipoprotein distributions for cholesterol and triglycerides by HPLC; analysis of plasma and tissue lipid compositions using enzymatic assays for major lipid classes; analysis of lipid fatty acid compositions of major lipid classes by GLC; analysis of cholesterol and cholesteryl ester concentrations by GLC; leukotriene and HETE analyses by HPLC. The technology available in this core laboratory will greatly facilitate the research in each of the projects.