The impact of environmental conditions on the prevalence and aerosol transmission of Streptococcus pyogenes

本项目将重点研究S.化脓菌最适合空气中生存，正在进行的MRC项目中进行鉴定和分离。该项目将利用现有的技术和开发新的方法来调查影响空气中生存的环境因素，并利用实地调查来了解和告知潜在的加强爆发控制指南。目的：在ICL三级实验室建立CELEBS仪器，验证S.化脓菌的产生、悬浮和捕获能力适应当前的生物气溶胶产生室，以控制环境条件（T & RH），并将稳定性和培养形成潜力与CELEBS进行比较适应CELEBS和生物气溶胶室，以促进共同暴露于气体和气溶胶污染源。化脓菌的生存能力与氧化性气体（NO2，O3），有机和无机气溶胶的环境浓度的变化。与UKHSA进行现场工作，对生物气溶胶进行采样，培养并与实验室菌株进行比较。在伦敦帝国理工学院建立CELEBS仪器将大大提高实验室评估影响沙门氏菌生存能力的环境因素的能力。此外，通过将其与已建立的实验室方法进行比较，并加强两者以研究更广泛的大气因素的影响，我们希望深入了解S.化脓性疾病爆发。这与学期时间无关，而是发生在春季;这表明潜在的环境影响。通过将爆发发生的条件与实验室调查联系起来，有机会了解环境条件，并为爆发管理提供最新指南。

This project will focus on genetic strains of S. pyogenes best adapted to airborne survival which are being identified and isolated during an ongoing MRC project. This project will utilise established techniques and develop new approaches to investigate the environmental factors which influence survival in air, and use fieldwork to contextualise and inform potential enhanced outbreak control guidelines. Objectives:Establish CELEBS instrument in ICL category 3 laboratory and validate stability of S. pyogenes generation, levitation and capturabilityAdapt current Bioaerosol Generation Chamber to allow control of environmental conditions (T & RH) and compare stability and culture forming potential to CELEBSAdapt CELEBS and biological aerosol chamber to facilitate co-exposure to gaseous and aerosol pollution sourcesInvestigate S. pyogenes survivability with variations in ambient concentrations of oxidising gases (NO2, O3), organic and inorganic aerosols.Undertake field work with UKHSA to sample bioaerosols, culture and compare to laboratory strains. Assess environmental conditions prevailing during outbreaks to contextualise laboratory experiments.Establishing the CELEBS instrument at Imperial College London will significantly enhance the capabilities of the laboratory to assess the environmental factors that influence survivability of S. pyogenes and other airborne infectious diseases.Furthermore, by comparing this with the established laboratory approach, and enhancing both to investigate the influence of a broader range of atmospheric factors, we hope to gain insight into the established periodicity of S. pyogenes outbreaks. This are not correlated with term times, and instead occur in spring time; this indicates a potential environmental influence.By relating the conditions in which outbreaks occur to the laboratory investigation, there is an opportunity to contextualise the environmental conditions and provide insight into updated guidelines for outbreak management.