Analytical and Phenotyping Core

核心C：分析和表型分析核心 分析核心的目的是提供最先进的交感神经生化分析 功能，包括儿茶酚胺、其代谢物、去甲肾上腺素清除和 溢出效应。这一资源对于我们的新团队的认可和描述至关重要 涉及自主神经系统的疾病，如多巴胺-β-羟基酶缺乏症。一个 在这个核心中建立了各种技术来支持单个项目，包括 血浆体积和动态液体位移的测定。所有四个项目都将利用这一资源。 交感神经活动的生化评估将补充其他自主神经评估方法 在《临床核心》中有详细介绍。此外，我们还实施了动物表型鉴定设施，使 开发了评估人类和小鼠自主神经功能的方法。这其中包括， 监测清醒无限制小鼠动脉内压，监测肾交感神经 活动和高保真的心电图，在完整和去鼻窦神经的小鼠。转基因小鼠 在翻译自主神经知识方面变得越来越重要，这个表型小鼠实验室将 在这方面至关重要。

Core C: Analytical and Phenotyping Core The purpose of the Analytical Core is to provide state-of -the art biochemical analysis of sympathetic function, including measurements of catecholamines, their metabolites, and norepinephrine clearance and spillover. This resource has been critical to the recognition and characterization by our group of new disorders involving the autonomic nervous system, such ads dopamine-beta-hydroxylase deficiency. A variety of techniques have been established in this Core to support individual projects, including the determination of plasma volume and dynamic fluid shifts. All four projects will utilize this resource. Biochemical evaluation of sympathetic activity will complement other methods of autonomic evaluation detailed in the Clinical Core. In addition, we have implemented an animal phenotyping facility, adapting the methods developed to evaluate autonomic function in humans, to the mice. This includes, among others, monitoring intraarterial blood pressure in awake unrestricted mice, monitoring renal sympathetic nerve activity and high fidelity ECG, in intact and sinoartic-denervated mice. Genetically-modified mice are increasingly important in translating autonomic knowledge, and this phenotyping mice laboratory will be essential in this regard.