Core--Cellular and Molecular Biology

该核心将促进在计划项目的所有项目中使用最先进的细胞和分子生物学技术。核心的职责将涉及两个独立但相关的职能：1）组织培养单位将提供组织培养细胞和完成单个提案中的细胞生物学方案所需的专业知识; 2）分子生物学单位将提供试剂和专业知识，以完成五个项目中描述的分子生物学实验。下文描述了核心中使用的主要方法的具体细节。这些包括但不限于 逆转录酶-聚合酶链反应、基因阵列杂交、北方、南方和西方印迹分析、反义寡核苷酸和siRNA的开发、重组腺病毒构建体的产生、用于核糖探针开发的基因片段的亚克隆、核糖探针合成和启动子分析。如果需要，将在拟定研究过程中向研究者提供这些程序，并根据需要开发新的方法。

This core will facilitate the use of state-of-the-art cellular and molecular biology technologies in all of the projects in the Program Project. The responsibilities of the core will involve the performance of two separate but related functions: 1) The Tissue Culture unit will provide tissue culture cells and the expertise necessary to complete the cellular biology protocols in the individual proposals and 2) The Molecular Biology unit will provide reagents and expertise to complete the molecular biology experiments described in the five projects. Described below are specific details of the primary methods used in the Core. These include, but are not limited to, reverse transcriptase-polymerase chain reaction, gene array hybridization, Northern, Southern and Western blot analysis, development of antisense oligonucleotides and siRNAs, generation of recombinant adenoviral constructs, subcloning of gene fragments for riboprobe development, and riboprobe synthesis, and promoter analysis. If required, these procedures will be provided to the investigators during the course of the proposed studies and new methodologies will be developed as needed.