Core B-Genetic Models and Phenotyping Core

这个核心是这个项目中所有转基因和基因敲除实验的核心。将提供的服务 由核心提供的资金将包括产生转基因、敲入和敲除动物、基因分型 并为项目3将动物运送给莱德尔博士。核心还将为个人提供支持 项目中的鼠标模型的初始角色化。每种鼠标模型的特征将 包括仔细分析转基因的表达模式，以及病理和生理 由于转基因表达而发生的变化。在过去5年的授权期内，核心 每年管理300多个小鼠笼子，协助所有新的转基因和 并对小鼠进行生理测量。 该核心将在以下领域为PPG提供支助： A.遗传小鼠模型的制备和维护 B.转基因、敲入和敲除小鼠的特征，包括组织的制备和分析 动物标本 C.基线生理测量，包括血流动力学和超声心动图 D.用于药物测试的转基因和基因敲除小鼠的生理测量。 E.在自由放养的WT和转基因小鼠中进行VT的非侵入性检测 活体小鼠心脏的电生理学研究。 心电图和血压记录。

This core is central for all transgenic and knockout experiments in this program project. Services that will be provided for by the core will include the generation of transgenic, knock-in and knockout animals, genotyping and transporting animals to Dr. Lederer for Project 3. The core will also provide support for the individual projects in the initial characterization of mouse models. The characterization of each mouse model will include careful analysis of the expression pattern of the transgene, and pathological and physiological changes, which occur as a result of transgene expression. In the past 5 years of the grant period the core managed over 300 cages of mice per year, assisted in pathological analysis of all new transgenic and knockout mice and performed physiological measurements on the mice. The core will provide support for the PPG in the following areas: A. The preparation and maintenance of genetic mouse models B. Characterization of transgenic, knock-in and knock-out mice including preparation and analysis of tissue specimens from animals C. Baseline physiological measurements including hemodynamics and echocardiography D. Physiological measurements on transgenic and knockout mice for drug testing. E. Non-invasive testing for VT in free ranging WT and transgenic mice F. Electrophysiological studies on the in vivo murine heart. G. Electrocardiogram and blood pressure recordings.