Applied Biosystems 7900HT Fast Real-Time PCR System

描述(由申请人提供)：这项NCRR共享仪器提案寻求资金，为威尔医学院(WMC)的微阵列核心设施(MCF)升级应用生物系统7900HT快速实时聚合酶链式反应系统。目前的实时聚合酶链式反应机器(Taqman7700)安装于1997年，已被WMC的研究人员大量使用来验证他们的微阵列数据。然而，该系统存在严重缺陷，包括工作站不稳定，导致频繁的崩溃，从而导致整个PCR反应失败；它的运行时间也很长，限制了每天的使用次数。这些缺陷正在阻碍WMC的研究。升级后的7900HT系统具有改进的高性能能力，包括用户可互换的块格式、快速聚合酶链式反应选项和384井TaqMan低密度阵列。7900HT将是WMC独有的宝贵资源，它是一种实时定量聚合酶链式反应系统，功能范围更广，处理能力更高，处理更快、更简单、更准确，比目前学院所有的实时聚合酶链式反应仪器都要好。这些改进对于基础和临床研究人员验证微阵列数据至关重要；它们对于WMC正在进行的高通量功能基因组学、突变和缺失研究也是必不可少的。这要求升级到最先进的应用生物系统7900HT快速实时聚合酶链式反应系统，这将极大地提高MCF的服务质量和数量，以支持WMC由NIH资助的一系列项目。目前由NIH资助的30名WMC教职员工聚集在一起，加强MCF的这些服务，他们的研究项目的成功内在地依赖于这些服务。

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for upgrading an Applied Biosystems 7900HT Fast Real-Time PCR System for the Microarray Core Facility (MCF) of Weill Medical College (WMC). The current real-time PCR machine (Taqman 7700), installed in 1997, has been heavily used by WMC's investigators to validate their microarray data. However, this system has severe defects, including the instability of the workstation, which causes frequent crashes and thereby the failure of entire PCR reactions; it also has a long running time, which limits the number of uses per day. These defects are hampering research at WMC. The upgraded 7900HT system has an improved capability of high-performance, which includes user-interchangeable block formats, a Fast PCR option and a 384-well TaqMan Low Density Array. The 7900HT will be a unique and valuable resource at WMC as a real-time quantitative PCR system with a greater range of functions, higher throughput capacity, faster, simpler and more accurate processing than all current real-time PCR instruments in the college. These improvements are critical to the validation of microarray data for basic and clinical researchers; they are also essential for high throughput functional genomics, mutation and deletion studies that are being carried out at WMC. This requested upgrade to the state-of-the-art Applied Biosystems 7900HT Fast Real-Time PCR System will greatly enhance the quality and quantity of the services at MCF in support of a wide range of NIH-funded projects at WMC. Thirty WMC faculty members, who are currently supported by NIH funding, have come together to strengthen these services of MCF, on which the success of their research projects inherently depend.