15T ULTRAHIGH FIELD HORIZONTAL MR MICROSCOPE FOR MOUSE IMAGING: CARDIOVASCULAR

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：要求部分资金用于15特斯拉110毫米内径水平孔磁铁系统，作为独特的最先进的MR扫描仪设施的中心组件，该设施将服务于nih资助的大型地理上不同的用户社区，包括各种生物医学学科。我们目前为MGH/哈佛/麻省理工学院系统内以及大波士顿/剑桥地区的用户（我们自己的调查员，合作者和外部研究人员）提供服务。然而，目前的用户没有足够的磁体通道，许多其他人也想利用我们的设施，但不能轻易这样做：我们目前的小口径（非临床）磁共振仪器是超负荷的，在许多情况下是24x7运行的。所要求的设备将极大地扩展我们为当地nih资助的用户社区服务的能力，并为用户群提供重要的新功能。特别是，提议的高场水平磁铁将消除传统垂直极高场磁铁在垂直位置保持动物（特别是那些生理受损的动物）存活和健康的严重限制；为EPI、扩散和高空间分辨率研究提供快速而强大的梯度（-600 mT/m， -100 us上升时间，在我们的任何水平系统上都不可用）；稳健性，可靠性，便捷性和高通量的临床控制台，以及卓越的信噪比，化学位移色散和敏感性为基础的对比非常高的磁场强度。服务的机构包括MGH（放射科、心脏科、麻醉科和内科）、儿童医院（骨科）、波士顿大学医学院（内科）、塔夫茨大学（生物医学工程）和贝斯以色列女执事医疗中心（骨科和肿瘤科）。项目包括心脏肥厚和衰竭的研究，心脏干细胞的追踪，NO合成酶和心室重构，NO合成酶和动脉粥样硬化，吸入NO和麻醉，针灸的神经机制，多巴胺介导的神经血管偶联，基于铁氧体纳米颗粒的fMRI对比，灌注成像，骨矿化的MR测量，糖尿病和t淋巴细胞，组织工程和生物反应器开发，以及中枢神经系统损伤的基因修复

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Partial funding is requested for a 15 Tesla 110 mm inside diameter horizontal bore magnet system to be the central component in a unique state-of-the-art MR scanner facility that will serve a large NIH-funded geographically diverse user community encompassing a wide variety of biomedical disciplines. We currently serve users (our own investigators, collaborators and outside researchers) both within the MGH/Harvard/MIT system as well as from around the greater Boston/Cambridge region. However, current users do not get sufficient magnet access, and many others would like to take similar advantage of our facilities, but cannot readily do so: our current small-bore (nonclinical) MR instruments are overscheduled, in many cases operating on a 24x7 basis. The requested equipment would greatly expand our capacity to serve the local NIH-funded user community, as well as offer important new features to the user base. In particular, the proposed high field horizontal magnet would eliminate the serious limitations associated with keeping animals (especially those with compromised physiology) alive and well in the vertical position in conventional vertical very high field magnets; provide fast and powerful gradients (-600 mT/m, -100 us risetime, unavailable on any of our horizontal systems) for EPI, diffusion and high spatial resolution studies; the robustness, reliability, convenience and high throughput of a clinical console, and extraordinarily high magnetic field strength for outstanding signal-to-noise ratio, chemical shift dispersion and susceptibility- based contrast. The institutions to be served include MGH (radiology, cardiology, anesthesia and medicine), Children's Hospital (orthopedic surgery), Boston University School of Medicine (medicine), Tufts University (biomedical engineering), and Beth Israel Deaconess Medical Center (orthopedic surgery and oncology). Projects include studies of cardiac hypertrophy and failure, tracking of cardiac stem cells, NO synthase and ventricular remodeling, NO synthase and atherosclerosis, inhaled NO and anesthesia, neural mechanisms of acupuncture, dopamine mediated neurovascular coupling, ferrite nanoparticle-based contrast in fMRI, perfusion imaging, MR measurement of bone mineralization, diabetes and T-lymphocytes, tissue engineering and bioreactor development, and gene repair in CNS injur