15T ULTRAHIGH FIELD HORIZONTAL MR MICROSCOPE FOR MOUSE IMAGING: CARDIOVASCULAR

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请者提供)：申请部分资金用于15特斯拉110毫米内径水平孔磁体系统，作为独特的最先进磁共振扫描仪设施的中心组件，该设施将服务于由NIH资助的涵盖各种生物医学学科的大型地理多样化用户社区。我们目前在麻省理工学院/哈佛/麻省理工学院系统内以及大波士顿/剑桥地区为用户(我们自己的研究人员、合作者和外部研究人员)提供服务。然而，目前的用户没有获得足够的磁铁接入，许多其他人也想利用我们的设施，但无法轻易做到：我们目前的小口径(非临床)磁共振仪器计划超时，在许多情况下是全天候运行的。所要求的设备将极大地扩大我们为当地NIH资助的用户群提供服务的能力，并为用户群提供重要的新功能。特别是，拟议的高场水平磁铁将消除传统垂直甚高场磁铁中保持动物(尤其是那些生理受损的动物)在垂直位置存活所面临的严重限制；为计划免疫、扩散和高空间分辨率研究提供快速而强大的梯度(-600 mt/m，-100 us上升时间，在任何我们的水平系统上都不具备)；临床控制台的稳健性、可靠性、便利性和高通量；以及出色的信噪比、化学位移弥散和基于敏感度的对比度的极高磁场强度。将接受服务的机构包括MGH(放射学、心脏病、麻醉和医学)、儿童医院(整形外科)、波士顿大学医学院(医学)、塔夫茨大学(生物医学工程)和贝丝以色列女执事医疗中心(整形外科和肿瘤学)。项目包括心肌肥厚和衰竭的研究，心肌干细胞的追踪，一氧化氮合酶与心室重构，一氧化氮合酶与动脉粥样硬化，吸入一氧化氮与麻醉，针刺的神经机制，多巴胺介导的神经血管偶联，铁酸盐纳米颗粒在功能磁共振成像中的对比，灌注成像，骨矿化的磁共振测量，糖尿病和T淋巴细胞，组织工程和生物反应器的开发，以及中枢神经系统损伤的基因修复

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Partial funding is requested for a 15 Tesla 110 mm inside diameter horizontal bore magnet system to be the central component in a unique state-of-the-art MR scanner facility that will serve a large NIH-funded geographically diverse user community encompassing a wide variety of biomedical disciplines. We currently serve users (our own investigators, collaborators and outside researchers) both within the MGH/Harvard/MIT system as well as from around the greater Boston/Cambridge region. However, current users do not get sufficient magnet access, and many others would like to take similar advantage of our facilities, but cannot readily do so: our current small-bore (nonclinical) MR instruments are overscheduled, in many cases operating on a 24x7 basis. The requested equipment would greatly expand our capacity to serve the local NIH-funded user community, as well as offer important new features to the user base. In particular, the proposed high field horizontal magnet would eliminate the serious limitations associated with keeping animals (especially those with compromised physiology) alive and well in the vertical position in conventional vertical very high field magnets; provide fast and powerful gradients (-600 mT/m, -100 us risetime, unavailable on any of our horizontal systems) for EPI, diffusion and high spatial resolution studies; the robustness, reliability, convenience and high throughput of a clinical console, and extraordinarily high magnetic field strength for outstanding signal-to-noise ratio, chemical shift dispersion and susceptibility- based contrast. The institutions to be served include MGH (radiology, cardiology, anesthesia and medicine), Children's Hospital (orthopedic surgery), Boston University School of Medicine (medicine), Tufts University (biomedical engineering), and Beth Israel Deaconess Medical Center (orthopedic surgery and oncology). Projects include studies of cardiac hypertrophy and failure, tracking of cardiac stem cells, NO synthase and ventricular remodeling, NO synthase and atherosclerosis, inhaled NO and anesthesia, neural mechanisms of acupuncture, dopamine mediated neurovascular coupling, ferrite nanoparticle-based contrast in fMRI, perfusion imaging, MR measurement of bone mineralization, diabetes and T-lymphocytes, tissue engineering and bioreactor development, and gene repair in CNS injur