15T ULTRAHIGH FIELD HORIZONTAL MR MICROSCOPE FOR MOUSE IMAGING: CARDIOVASCULAR

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：部分资金要求用于15特斯拉110 mm内径水平孔磁体系统，该系统将成为独特的最先进MR扫描仪设施的核心组件，该设施将服务于NIH资助的地理位置多样化的大型用户社区，包括各种生物医学学科。我们目前为用户（我们自己的研究人员，合作者和外部研究人员）提供服务，包括MGH/哈佛/麻省理工学院系统以及大波士顿/剑桥地区。然而，目前的用户无法获得足够的磁体访问，许多其他用户希望利用我们的设施，但无法轻易做到：我们目前的小口径（非临床）MR仪器计划过多，在许多情况下，24 x7全天候运行。所要求的设备将大大扩展我们为当地NIH资助的用户社区提供服务的能力，并为用户群提供重要的新功能。特别是，建议的高场水平磁体将消除与饲养动物相关的严重限制（尤其是那些生理机能受损的患者）在传统垂直极高磁场磁体中的垂直位置上保持良好的生命力;提供快速而强大的梯度（-600 mT/m，-100 us上升时间，在我们的任何水平系统上都不可用），用于EPI、扩散和高空间分辨率研究;临床控制台的坚固性、可靠性、便利性和高吞吐量，以及极高的磁场强度，以实现出色的信噪比、化学位移分散和基于磁化率的对比度。提供服务的机构包括MGH（放射科、心脏科、麻醉科和内科）、儿童医院（矫形外科）、波士顿大学医学院（医学）、塔夫茨大学（生物医学工程）和贝斯以色列女执事医疗中心（矫形外科和肿瘤科）。项目包括心脏肥大和衰竭的研究，心脏干细胞的跟踪，NO合酶和心室重塑，NO合酶和动脉粥样硬化，吸入NO和麻醉，针灸的神经机制，多巴胺介导的神经血管偶联，功能磁共振成像中基于铁氧体纳米颗粒的对比，灌注成像，骨矿化的MR测量，糖尿病和T淋巴细胞，组织工程和生物反应器开发，中枢神经系统损伤的基因修复

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Partial funding is requested for a 15 Tesla 110 mm inside diameter horizontal bore magnet system to be the central component in a unique state-of-the-art MR scanner facility that will serve a large NIH-funded geographically diverse user community encompassing a wide variety of biomedical disciplines. We currently serve users (our own investigators, collaborators and outside researchers) both within the MGH/Harvard/MIT system as well as from around the greater Boston/Cambridge region. However, current users do not get sufficient magnet access, and many others would like to take similar advantage of our facilities, but cannot readily do so: our current small-bore (nonclinical) MR instruments are overscheduled, in many cases operating on a 24x7 basis. The requested equipment would greatly expand our capacity to serve the local NIH-funded user community, as well as offer important new features to the user base. In particular, the proposed high field horizontal magnet would eliminate the serious limitations associated with keeping animals (especially those with compromised physiology) alive and well in the vertical position in conventional vertical very high field magnets; provide fast and powerful gradients (-600 mT/m, -100 us risetime, unavailable on any of our horizontal systems) for EPI, diffusion and high spatial resolution studies; the robustness, reliability, convenience and high throughput of a clinical console, and extraordinarily high magnetic field strength for outstanding signal-to-noise ratio, chemical shift dispersion and susceptibility- based contrast. The institutions to be served include MGH (radiology, cardiology, anesthesia and medicine), Children's Hospital (orthopedic surgery), Boston University School of Medicine (medicine), Tufts University (biomedical engineering), and Beth Israel Deaconess Medical Center (orthopedic surgery and oncology). Projects include studies of cardiac hypertrophy and failure, tracking of cardiac stem cells, NO synthase and ventricular remodeling, NO synthase and atherosclerosis, inhaled NO and anesthesia, neural mechanisms of acupuncture, dopamine mediated neurovascular coupling, ferrite nanoparticle-based contrast in fMRI, perfusion imaging, MR measurement of bone mineralization, diabetes and T-lymphocytes, tissue engineering and bioreactor development, and gene repair in CNS injur