Immmunogenicity and Structure of trimeric HIV-1 gp120 gl

在这项研究中，我们生产了HIV-1Env gp120受体结合域的可溶性版本，该结合域来自由异源三聚基序稳定的初级分离物(Gcn4)。我们已经生产了毫克量的这些蛋白质，并进行了详细的生化和生物物理分析。我们已经在兔子身上测试了这些糖蛋白的免疫原性。中和的广度表明，这些糖蛋白是有趣的平台，可以进一步修饰以更好地诱导广泛的中和抗体。 这项研究的第二个目标是获得这些三聚体HIV-1gp120糖蛋白的晶体结构。潘切拉博士将对这些蛋白质以及她在怀亚特实验室生产的全长gp120糖蛋白进行结构研究，这是她博士研究的一部分(2005年4月完成)。

In this study, we have produced soluble versions of just the HIV-1 Env gp120 receptor binding domain derived from a primary isolate stabilized by heterologous trimerization motifs (GCN4). We have produced milligram quantities of these proteins and and performed a detailed biochemical and biophysical analysis. We have tested these glycoproteins for immunogenicity in rabbits . Breadth of neutralization indicate that these glycoproteins are interesting platforms for further modification to better elicit broadly neutralizing antibodies. A second goal of this study is to obtain crystal structures of these trimeric HIV-1 gp120 glycoproteins. Dr. Pancera will conduct structural studies on these proteins as well as full length gp120 glycoproteins that she has produced in the Wyatt laboratory as part of her doctoral studies (completed April 2005).