Rapid imaging of single-photon and fluorescence lifetime in living tissue

新的先进成像技术，如单光子雪崩二极管（SPAD）阵列，可以通过荧光详细测量活组织发射的单个光子。这包括通过FLIM-FRET等技术测量荧光寿命、分子环境的敏感探针和活细胞中存在的机械力。SPAD阵列被用于汽车和移动的电话的距离测量，并且它们开始被用于生物医学成像。学生将解决以下挑战：-研究新的光学方法，以提高成像系统的有效分辨率，在生物样品内的更大视野内成像-将SPAD技术与先进的显微镜技术相结合，如光片，共聚焦和结构化照明成像，以提高FLIM-FRET成像的性能。成像运动细胞的生理变化，如斑马鱼幼虫中的免疫细胞。学生将设计和构建新的光学和计算系统来应对这些挑战，与硬件制造商合作，充分利用尖端技术，并与生物研究人员合作，将我们的研究应用于现实世界的问题。

New advanced imaging technologies such as single-photon avalanche diode (SPAD) arrays allow detailed measurements of individual photons emitted by living tissue via fluorescence. This includes measurements of fluorescence lifetimes, a sensitive probe of the molecular environment and mechanical forces present in living cells via techniques such as FLIM-FRET. SPAD arrays are finding uses for range measurements in cars and mobile phones, and they are beginning to see use for biomedical imaging. The student will tackle the following challenges:- Researching new optical approaches to increase the effective resolution of the imaging system, to image across a larger field of view within biological samples- Combining the SPAD technology with advanced microscopy techniques such as light sheet, confocal and structured illumination imaging, for improved performance of FLIM-FRET imaging.- Imaging physiological changes to motile cells such as immune cells in zebrafish larvae.The student will design and construct new optical and computational systems to tackle these challenges, collaborating with hardware manufacturers to get the most out of cutting-edge technologies, and collaborating with biological researchers to apply our research to real-world problems.