In-field Detection Instrument for Rapid Screening of Avian Influenza Viruses

描述(由申请人提供)： 这个第一阶段的项目建议使用正在申请专利的基于生物纳米棒的样品制备程序来研究禽流感病毒的捕获和浓缩，并使用获得专利的毛细管免疫传感器对捕获的病毒进行特定检测。这种新的样本制备技术可以从几乎任何样本来源：口咽拭子、粪便或血液中分离和浓缩目标病毒。在第二阶段，样本预处理和生物传感器检测将完全整合到现场测试设备中，以快速筛查家禽群中的禽流感感染。目前迫切需要进行快速现场分析，以便能够快速隔离AI阳性禽群，以防止病毒进一步传播。然而，没有一种竞争的人工智能检测技术能像这里提出的那样，设想出一种从样本采集到人工智能病毒筛选的完整的现场检测仪器。拟议的检测仪器将完全自动化，可以在禽舍之间运输，以评估单个鸡群的人工智能状态。这一设置将允许一名技术人员在一天内对多个羊群进行筛选，并可在运输供屠宰之前纳入羊群收集程序。整个系统的便携性将允许在偏远地区监测商业和家庭家禽群，并将彻底改变监测和控制致病性禽流感病毒传播和突变的能力。这种设备的开发将使家禽生产经理能够迅速遵守世界动物卫生组织(OIE)关于商业家禽作业中人工智能识别的标准。

DESCRIPTION (provided by applicant): This Phase I project proposes to investigate the capture and concentration of avian influenza (AI) viruses using a patent-pending, bio-nanobead based sample preparation procedure and specific detection of the captured viruses with a patented capillary immunosensor. The novel sample preparation technique may allow for isolation and concentration of a target virus from almost any sample source: oropharyngeal swabs, droppings or blood. In Phase II, the sample pretreatment and the biosensor assay will be fully integrated into an in-field testing device for rapid screening of AI infection in poultry flocks. There is an urgent need for rapid on-site analysis that allows an AI-positive flock to be quickly quarantined to prevent further spread of the virus. However, none of the competing AI detection technologies have envisioned an in-field detection instrument that is complete from sample acquisition to AI virus screening as is proposed here. The proposed testing instrument will be fully automated and can be transported between poultry houses to assess the AI status of individual flocks. This set up would allow a single technician to screen multiple flocks within one day and could be incorporated into the flock collection procedure prior to transport for slaughter. The portability of the complete system would allow both commercial and family flocks of domestic birds to be monitored in remote locations and would revolutionize the ability to monitor and control the spread and mutation of pathogenic AI viruses. The development of this device would allow poultry production managers to rapidly comply with OIE (World Organization for Animal Health) standards regarding the identification of AI in commercial poultry operations.