Next generation cell imaging: developing new genetically encodable super-scattering Raman probes

细胞生物成像的进展已被证明是提高我们对生物过程的理解的关键。随着荧光蛋白(FP)允许对细胞内的特定目标进行遗传标记和跟踪，荧光显微镜作为“去”细胞成像方法应运而生。然而，也有一些缺点，比如光漂白和可以同时监测的目标数量有限。拉曼显微镜有可能克服这些限制，因为拉曼散射可以是光谱窄和可调的，以及光稳定的，但需要开发基因编码的探测器。

Advances in cellular bioimaging have proved pivotal in improving our understanding of biological processes. Fluorescence microscopy emerged as the "go to" cell imaging approach as fluorescent proteins (FPs) allowed genetic tagging and tracking of specific targets inside a cell. However, there are disadvantages such as photobleaching and the limited number of targets that can be monitored simultaneously. Raman microscopy has the potential to overcome these limitations since Raman scattering can be spectrally narrow and tuneable, as well as photostable, but requires the development of genetically encoded probes.