A State-of-the-art BIACORE for UCSF Mission Bay

描述(由申请人提供)：加州大学旧金山分校任务湾项目最先进的Biacore项目摘要/摘要我们正在请求一台Biacore T100仪器，为加州大学旧金山分校的多名研究人员提供使用表面等离子体共振(SPR)技术对蛋白质-蛋白质和蛋白质-配体相互作用进行量化分析的能力。加州大学旧金山分校不断扩大的使命湾园区目前唯一可用的SPR仪器是Biacore 1000。实验需求和使用都在增长，因此B1000是不够的。随着加州大学旧金山分校任务湾校区研究设施的快速扩张，有必要获得第二台更强大的仪器。Biacore T100改进了灵敏度、温度控制、自动化和易用性，是对当前Biacore 1000的重大改进。它将使高通量采样和结合热力学分析等实验方法成为可能，而这些是Biacore 1000无法实现的。与目前的Biacore 1000相补充，T100将简化SPR仪器的培训和使用，以便更多的研究人员熟悉这项技术，并可能计划在他们的工作中使用它。Biacore T100的加入将使加州大学旧金山分校的一大批科学家受益，并使调查人员能够充分利用SPR技术的最大能力。该提案的主要用户代表10个不同的部门，包括加州大学旧金山分校综合癌症中心、定量生化研究所、加州大学旧金山分校糖尿病中心和格莱斯顿研究所的附属机构。生物分子结合相互作用的表征是所有这些研究人员研究计划的核心，所有这些都将受益于T100的特殊敏感性和高通量能力。调查人员致力于共享该仪器，并将积极主动地将其提供给其他用户。这将包括向仪器的新用户或临时用户提供高技能核心用户的服务。T100将极大地促进正在进行的、由美国国立卫生研究院资助的基础研究，这些研究共享基本的长期目标，即了解构成生物和医学相关相互作用基础的化学和物理原理。与公共卫生药物发现的相关性取决于对人类疾病机制的了解。开发候选药物的化学合成策略依赖于准确测量它们与蛋白质靶标的结合。这项建议中描述的设备在为科学家提供最灵敏的工具来检测疾病机制中的生物蛋白质相互作用以及定量测量候选药物与目标蛋白质的结合方面是独一无二的。

DESCRIPTION (provided by applicant): A State-of-the-Art BIACORE for UCSF Mission Bay Project Summary / Abstract We are requesting a Biacore T100 instrument to provide multiple investigators at UCSF the capability of quantifying the analyses of protein-protein and protein-ligand interactions using surface plasmon resonance (SPR) technology. The only currently available SPR instrument at UCSF's expanding Mission Bay campus is a Biacore 1000. Experimental demands and usage have grown so that the B1000 is inadequate. With the fast rate of expansion of research facilities at the UCSF Mission Bay campus, it is necessary to secure a second and more capable instrument. The improved sensitivity, temperature control, automation and ease of use afforded by the Biacore T100 represents a significant enhancement over the current Biacore 1000. It will enable experimental approaches such as high-throughput sampling and analysis of binding thermodynamics that are impossible with the Biacore 1000. Complemented by the current Biacore 1000, the T100 will ease training and the use of SPR instruments so that more researchers will become familiar with the technology and may plan using it in their work. The addition of a Biacore T100 will benefit a large community of scientists at UCSF and allow investigators to exploit the fullest capabilities of SPR technology. The major users on this proposal represent ten different departments and include affiliations with the UCSF Comprehensive Cancer Center, the Institute for Quantitative Biochemical Research, the UCSF Diabetes Center, and the Gladstone Institutes. Characterization of biomolecular binding interactions is central to the research programs of all these investigators, and all will benefit from the exceptional sensitivity and high throughput capacity of the T100. The investigators are committed to sharing the instrument and will be proactive in making it available for other users. This will include providing new or occasional users of the instrument with access to the services of highly skilled core users. The T100 will greatly facilitate ongoing, NIH-funded basic investigations that share the fundamental long-term goal of understanding the chemical and physical principles that underlie biologically and medically relevant interactions. Relevance to Public Health Drug discovery depends on knowing mechanisms of human disease. Developing strategies for the chemical synthesis of candidate drugs depends on accurate measurement of their binding to protein targets. The equipment described in this proposal is unique in providing scientists with the most sensitive tools for detecting biological protein interactions in disease mechanisms and for quantitative measurements of binding drug candidates to target proteins.