Purchase of LTQ-Orbitrap Hybrid Mass Spectrometer

描述（由申请人提供）：购买LTQ-轨道阱混合质谱仪本提案旨在为购买超高性能Thermo Electron LTQ-轨道阱混合MS提供资金。拟议的仪器对于UCI的许多NIH资助的研究项目至关重要，因为UCI校园内没有具有LTQ-轨道阱MS功能的质谱仪。该仪器同时提供高灵敏度、分辨率、质量准确度、动态范围和通量，这将增强我们对肽和蛋白质进行测序的能力，用于蛋白质鉴定和在阿托摩尔水平上对翻译后修饰进行全面表征。其目标是使用基于质谱的蛋白质组学方法来阐明包括癌症在内的各种人类疾病发展的分子机制。所要求的仪器将用于对26 S蛋白酶体复合物进行详细的结构-功能分析（黄博士），泛素化信号级联（Kaiser博士），癌症中的调节蛋白逆转录病毒样核心颗粒（Sandmeyer博士），LEF/TCF -连环蛋白复合物（Waterman博士），免疫细胞离子通道信号传导（Cahalan博士），参与亨廷顿病的细胞因子（Marsh博士），C.白念珠菌（刘博士），RNA尿苷酰转移酶作为潜在的抗锥虫药物靶点（Aphasizhev博士），以及癌症中线粒体完整性的调节（Wallace博士）。新仪器的高性能将使人们有可能确定疾病相关的蛋白质复合物的组成和它们的翻译后修饰的常规基础上。为了确保最大限度的使用和适当的维护，仪器将存放在PI Huang博士的质谱实验室。PI在蛋白质组学/生物质谱领域拥有超过10年的经验。Cahalan博士将担任咨询委员会主席，负责对共同文书的使用提供总体指导。Burlingame博士将担任外部咨询委员会成员。黄博士将与研究人员讨论实验策略，监督性能优化和优先安排。她的工作人员将提供日常监督的使用，培训和维护。UCI已同意提供15万美元用于购买拟议的仪器，并为仪器的维护和仪器操作员提供额外的财政支持四年。这10名主要研究人员参与了26个NIH支持的研究项目，这些项目对与人类健康和疾病相关的蛋白质组学研究的尖端技术需求不断增长。所要求的工具将代表UCI医学和生物科学学院生物医学研究的重要进展，作为五个不同部门成员的共享跨学科资源。

DESCRIPTION (provided by applicant): Purchase of the LTQ-Orbitrap hybrid mass spectrometer This proposal seeks funding for the purchase of an ultra high performance Thermo Electron LTQ-Orbitrap hybrid MS. The proposed instrument is essential to many NIH funded research programs at UCI as there is no mass spectrometer with the capabilities of the LTQ-Orbitrap MS available on the UCI campus. This instrument provides high sensitivity, resolution, mass accuracy, dynamic range and throughput simultaneously, which will enhance our ability to sequence peptides and proteins for protein identification and comprehensive characterization of posttranslational modifications at the attomole level. The goal is to use mass spectrometry-based proteomics approaches to elucidate the molecular mechanisms underlying the development of various human diseases including cancers. The requested instrument will be used to carry out detailed structure-function analyses of the 26S proteasome complex (Dr. Huang), ubiquitination signaling cascades (Dr. Kaiser), regulatory proteins in cancer (Dr. Lee), retro-virus-like core particles (Dr. Sandmeyer), LEF/TCF -catenin complexes (Dr. Waterman), immune cell ion channel signaling (Dr. Cahalan), cellular factors involved in Huntington disease (Dr. Marsh), dimorphic switching and control of pathogenic forms of C. albicans (Dr. Liu), RNA uridylyltransferases as potential anti-Trypanosome drug targets (Dr. Aphasizhev), and regulation of mitochondria integrity in cancer (Dr. Wallace). The high performance of the new instrumentation will make it possible to determine the composition of disease-related protein complexes and their posttranslational modifications on a routine basis. To ensure maximum usage and proper maintenance, the instrument will be housed in the mass spectrometry laboratory of the PI, Dr. Huang. The PI has over 10 years experience in the area of proteomics/biological mass spectrometry. Dr. Cahalan will chair the Advisory Committee responsible for overall guidance for the shared instrument's use. Dr. Burlingame will serve as an external Advisory Committee member. Dr. Huang will discuss experimental strategies with investigators, oversee optimization of performance and prioritize scheduling. Her staff member will provide daily supervision of usage, training and maintenance. UCI has agreed to provide $150k for the purchase of the proposed instrument, and additional financial support for the instrument's maintenance and an instrument operator for four years. The ten principal Investigators are involved in 26 NIH-supported research programs with growing needs for cutting-edge technology for their proteomics research related to human health and diseases. The requested instrument would represent an important advance for biomedical research in the UCI Schools of Medicine and Biological Sciences as a shared interdisciplinary resource for members of five different departments.