Applied Biosystems 4800 MALDI TOF/TOF Mass Spectrometer

描述(由申请人提供)：肯塔基大学(英国)医学院的蛋白质组学核心设施申请416,290美元购买基质辅助激光解吸电离(MALDI)串联飞行时间(TOF/TOF)质谱仪。该应用程序的目标是提供先进的质谱学功能，以满足英国NIH资助的18名高生产率研究人员的蛋白质组学需求。与旧型号相比，应用生物系统4800 MALDI-TOF/TOF质谱仪的灵敏度提高了10倍，增强了前体离子选择，并利用高能(2keV)碰撞诱导解离对MALDI产生的单电荷前体离子进行了更有效的碎裂。其在MS和串联MS/MS模式下的卓越质量精确度和分辨率使强大的蛋白质组学能力成为可能。该设备将为蛋白质组学核心设施提供最先进的能力，并增强该设施满足不断增加的数量和强度的研究支持的能力。这种设备对于这些正在进行的研究计划以及在蛋白质鉴定、翻译后修饰表征、基因发现和功能蛋白质组学方面的新的合作研究计划是必不可少的。目前，英国或肯塔基州的任何大学都没有MALDI-TOF/TOF质谱仪。购买MALDI-TOF/TOF质谱仪对于继续扩大和加强英国在人类疾病和基础研究方面的蛋白质组学研究计划至关重要。正如本申请中所描述的，来自10个不同部门的18名NIH资助的研究人员对这台质谱仪有特定的需求，这将是一个共享的研究资源。事实上，由于校园内缺乏更灵敏和高通量的MALDI-TOF/TOF质谱仪，以及其他仪器目前的使用饱和，其中一些研究项目严重延误或受到影响。因此，购买MALDI-TOF/TOF质谱仪将对我们机构的研究能力产生重大影响。相关性这项由该仪器支持的研究涉及广泛的生物和生物医学研究，包括侧重于阿尔茨海默病、帕金森氏病和卢·格里克病等神经疾病的尖端项目；癌症生物学，包括前列腺癌、乳腺癌和皮肤癌的DNA修复、基因调控和细胞凋亡；以及糖尿病和心血管疾病。其他项目探索信号通路和基因转录调控的基本问题，这些问题对癌症、糖尿病和神经疾病等多种重大疾病的发病机制至关重要，或者研究金属蛋白酶在卵巢功能和女性健康中的作用。将该仪器添加到英国医学中心的蛋白质组学核心设施将显著提高这些研究计划的生产率和进展，从而推进NIH改善公共健康的使命。

DESCRIPTION (provided by applicant): The Proteomics Core Facility at the University of Kentucky (UK) College of Medicine requests $416,290 to acquire a matrix-assisted laser desorption ionization (MALDI) tandem time-of-flight (TOF/TOF) mass spectrometer. The goal of this application is to provide advanced mass spectrometry capabilities to meet the proteomics needs of 18 highly productive NIH-funded investigators at UK. Compared to an older model, the Applied Biosystems 4800 MALDI-TOF/TOF mass spectrometer offers 10x better sensitivity, enhanced precursor ion selection, and more efficient fragmentation of singly charged precursor ions generated by MALDI using high energy (2 KeV) collision-induced dissociation. Its superior mass accuracy and resolution in both MS and tandem MS/MS modes enable powerful proteomics capabilities. This equipment will provide state-of-the- art capabilities to the Proteomics Core Facility and enhance the ability of the facility to meet an ever-increasing volume and intensity of research support. This equipment is essential to these ongoing research programs and to stimulating and enabling new collaborative research initiatives on protein identification, posttranslational modification characterization, gene discovery, and functional proteomics. Currently, there is no MALDI- TOF/TOF mass spectrometer at UK or any universities in the Commonwealth of Kentucky. The purchase of the MALDI-TOF/TOF mass spectrometer is critical to the continuing expansion and enhancement of the proteomics research program in human disease and basic research at UK. As described in this application, 18 NIH-funded investigators from 10 different departments have specific needs for this mass spectrometer, which will be a shared research resource. Indeed, due to the lack of a more sensitive and high throughput MALDI- TOF/TOF mass spectrometer on campus and the current usage saturation of the other instruments, some of these research projects are seriously delayed or compromised. Thus, the purchase of the MALDI-TOF/TOF mass spectrometer will have a major impact on research capabilities at our institution. Relevance The research to be supported by this instrument spans a wide spectrum of biological and biomedical research, including cutting-edge programs focused on neurological disorders such as Alzheimer's disease, Parkinson's disease and Lou Gehrig's disease; cancer biology including DNA repair, gene regulation and apoptosis in prostate, breast and skin cancers; and diabetes and cardiovascular disease. Other programs explore fundamental questions in signaling pathways and gene transcription regulation that are critical to the pathogenesis of multiple major diseases such as cancer, diabetes and neurological disorders or study the role of metalloproteases in ovarian function and women's health. The addition of this instrument to the Proteomics Core Facility at UK Medical Center will significantly enhance the productivity and progress of these research programs, thus advancing the NIH mission to improve public health.