Johns Hopkins High Throughput Fluorescence Reader System

描述（由申请人提供）：许多生物过程，特别是神经系统中发生在毫秒级的生物过程，以快速的时间尺度发生。鉴定生物活性化合物或核酸以探测机械过程和开发治疗疾病的线索是推进和扩大约翰霍普金斯研究的重要方面。这些项目以及其他生物信号过程通常需要专门的仪器，昂贵但必要的试剂和独特的技术专长。这些先决条件阻止了单个实验室解决这些领域的重要问题。为了克服这一障碍，我们建议获得一个快速和高通量的荧光动力学阅读器系统。共享的仪器将被纳入已经运行的高通量化合物筛选设施ChemCORE。要获得的仪器与现有的专业知识相结合，提供了资源和服务，这是很难或不切实际的个别实验室自己完成。该仪器的使用将利用霍普金斯现有的机器人技术能力和化学图书馆，并将显著增强正在进行的研究计划，并刺激来自不同部门和校园的研究人员在拟议的核心项目中概述的新的研究方向。新的实验机会所提供的待收购的仪器补充，但不重复，现有的大学核心设施。核心项目负责人拥有领导研究项目和富有成效的合作的不同寻常的强大历史。他们的项目在提案中详细说明了生物医学科学不同领域的概念或临床重要问题，这些问题将基础研究和转化应用结合起来。因此，在ChemCORE内对这种仪器能力的资本投资既可以实现又可以扩大NIH资助项目的合作研究。针对疾病靶标的高通量化合物筛选的综合能力以及高端仪器对各个实验室的可及性的扩大将促进人类疾病的临床干预。PHS 398/2590（Rev.05/01

DESCRIPTION (provided by applicant): Many biological processes, especially those in the nervous systems that occur on the order of milliseconds, take place with rapid temporal scales. Identification of bioactive compounds or nucleic acids to probe mechanistic processes and development of leads for treating diseases are vital aspects to advancing and expanding research at Johns Hopkins. These projects as well as other biological signaling processes often require specialized instrumentation, expensive but necessary reagents and unique technical expertise. Such prerequisites prohibit individual labs from tackling important problems in these areas. To surmount this barrier, we propose to acquire a fast and high throughput fluorescence kinetics reader system. The shared instrument will be incorporated into already operating high throughput compound screen facility, ChemCORE. The combination of the to-be-acquired instrumentation with existing expertise provides both resources and services that are difficult or impractical for individual laboratories to accomplish on their own. The use of this instrument will leverage the existing robotics capability and chemical libraries at Hopkins and will significantly enhance on-going research programs and stimulate new directions of research as outlined in the proposed core projects by investigators from different departments and campuses. The new experimental opportunities offered by the to-be-acquired instrument complement, but do not duplicate, existing University Core facilities. The core project leaders have unusually strong histories leading research programs and productive collaborations. Their projects as detailed in the proposal address conceptually or clinically important questions in different areas of biomedical sciences that interface basic research and translational applications. Therefore, the capital investment of this instrumentation capability within ChemCORE both enables and expands collaborative research of NIH-funded projects. The combined capability of high throughput compound screens against disease targets and the broadening accessibility of high-end instrument to individual laboratories will facilitate clinical intervention of human diseases. PHS 398/2590 (Rev. 05/01