High Throughput Fractionation and Concentration System for MALDI-MS

描述（由申请人提供）：用于MALDI-MS的高通量分馏和浓缩系统该小型企业创新研究（SBIR）第II阶段项目旨在建立在第I阶段可行性研究中完成的成功工作的基础上，以优化和构建用于平行制备多达96个血清样品的原型系统，用于基质辅助激光解吸电离（MALDI）质谱（MS）。该系统称为MALDIPlex（tm）M5样品制备站，由台式仪器、图形用户界面（GUI）和一次性96孔测试卡片组成。MALDIPlex检测盒经过专门设计，允许通过一系列堆叠的薄层支持物从每个孔平行垂直分离。该系统允许集成的样品纯化，分馏，浓缩和浓缩-所有在一个单一的，易于使用的盒。使用该系统，样品可以在不到45分钟的时间内同时制备。没有其他系统提供该系统可实现的潜在吞吐量和数据质量。第二阶段的工作将集中在进一步开发和优化的定量和分馏协议，以及改进的系统设计（自动化）和方法，从盒表面的蛋白质识别。这项创新技术有可能在改善对癌症等复杂疾病的理解、诊断、预后和治疗方面发挥关键作用。如果成功，第二阶段将产生一个优化的样品制备系统，为第三阶段的全面生产和商业化做好准备。7. MALDI-MS的高通量分馏和浓缩系统使用质谱法寻找新的药物靶点和疾病标志物（生物标志物）对于改善疾病的理解、诊断、预后和治疗具有巨大的潜力。许多复杂的危及生命的疾病，如癌症，需要早期诊断，以最大限度地提高有效治疗。如果成功，这个II期研究项目将能够使用质谱法进行大规模的临床相关生物标志物发现，这有可能揭示新的、高度敏感的生物标志物，用于早期疾病诊断和预测治疗反应。

DESCRIPTION (provided by applicant): High Throughput Fractionation and Concentration System for MALDI-MS This Small Business Innovative Research (SBIR) Phase II project aims to build upon the successful work completed in the Phase I feasibility study to optimize and construct a prototype system for the preparation of up to 96 serum samples in parallel for matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). This system, called the MALDIplex(tm) M5 Sample Prep Station, consists of a benchtop instrument, a graphical user interface (GUI), and single-use 96-well cartridges. The MALDIplex cartridges are specially designed to permit parallel vertical separation from each well through a series of stacked thin layer supports. The system permits integrated sample purification, fractionation, concentration, and desalting - all in a single, easy to use cartridge. Using this system, samples may be prepared simulaneously in less than 45 minutes. No other system offers the potential throughput and data quality achievable with this system. Phase II efforts will focus on the further development and optimization of quantitation and fractionation protocols, as well as improved system designs (for automation) and methods for protein identification from the cartridge surface. This innovative technology has the potential to play a key role in improving the understanding, diagnosis, prognosis, and treatment of complex disease such as cancer. If successful, the Phase II will result in an optimized sample preparation system ready for full scale manufacturing and commercialization in Phase III. 7. Project Narrative High Throughput Fractionation and Concentration System for MALDI-MS The search of novel drug targets and disease markers (biomarkers) using mass spectrometry holds tremendous potential for improving the understanding, diagnosis, prognosis, and treatment of disease. Many complex, life threatening diseases, such as cancer, require early diagnosis to maximize effective treatment. If successful, this Phase II research project will enable large scale, clinically-relevant biomarker discovery using mass spectrometry, which has the potential of revealing new, highly sensitive biomarkers for early disease diagnosis and to predict treatment response.