Mechanisms of MeCP2 gene expression regulation

描述（由申请人提供）：本申请的目的是确定MeCP 2基因表达控制的转录后机制。 主要研究者将描述MeCP2的替代多聚腺苷酸化，该过程以组织和发育特异性方式确定3' UTR的大小。 她还将研究mRNA稳定性在MeCP2表达调控中可能发挥的作用。 这些研究将在各种细胞系中进行，特别关注神经细胞。 然后，研究人员将转向使用这些细胞系建立的体外系统，以研究通过替代聚腺苷酸化和差异RNA稳定性的调节机制。 这些研究代表了MeCP2基因表达研究的一个未充分探索的领域，可能对Rett综合征中观察到的组织特异性效应产生关键影响。

DESCRIPTION (provided by applicant): The goal of this application is to determine the post-transcriptional mechanisms of gene expression control of the MeCP2 gene. The principal investigator will delineate alternative polyadenylation of MeCP2, the process that determines the size of the 3' UTR in a tissue-and developmentally-specific fashion. She will also investigate the role that mRNA stability may play in MeCP2 expression regulation. These studies will be performed in vivo in a variety of cell lines with special attention to neural cells. The investigator will then turn to established in vitro systems using these cell lines in order to investigate mechanisms of regulation by alternative polyadenylation and differential RNA stability. These studies represent an under-explored area of research on MeCP2 gene expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome.