Imaging and Microscopy Core

核心搬进医学院研究大楼进入新的实验室空间与巩固 并将所有设备和服务集中到一个位置。 建立了一个成像和显微镜核心网站，其中包括： 可用于免疫组织化学与应用特定的例子;可下载的组织学协议; 交互式网络表单，用于向核心人员提交查询;以及详细的服务和价格表列表， 加上支持核心的出版物书目。 核心小组提供的服务： 除了标准的组织学支持外，核心还提供： <$中心人员的培训和咨询 * 有需要的人士可就各种组织固定方法获得谘询 抗原修复方法的帮助和故障排除 * 确定最佳条件的抗体检测 * 培养细胞的免疫荧光 * 电子显微镜组织制备 * 用于共聚焦显微镜的组织和细胞制备 * 用于免疫组织化学的抗体纯化 * 用于细胞凋亡研究的固定切片中的TUNEL染色 TUNEL和PCNA或Ki-67双色染色用于细胞凋亡/增殖指数 * 用于定位组织切片中mRNA的原位杂交（荧光和比色可视化） 图像分析。核心小组提供服务，协助调查人员获取图像和记录 使用我们的图像分析工作站。使用Nikon Eclipse E400系列显微镜，单个 图像由功能齐全的计算机工作站采集、处理和打印。Image-Pro Plus 软件和NIH Image J用于定量形态测量分析。 作为培训中心。核心培训了研究人员，博士前和博士后学生以及 U 54研究人员在免疫组织化学、原位杂交、图像分析和组织化学领域的技术人员， 分析和数据处理。

The Core moved into the College of Medicine Research Building into new laboratory space with consolidation and centralization of all equipment and services into one location. A website for the Imaging and Microscopy Core was implemented containing: a database of antibodies available for immunohistochemistry with application specific examples; downloadable histology protocols; interactive web-form to submit inquiries to Core personnel; and a detailed list of services and price schedules, plus a bibliography of publications supported the Core. Services provided by the Core: In addition to standard histology support, the Core provided: ¿ Training and consultation for Center personnel * Consultation of the various fixation methods for tissues was available to those who required assistance and trouble shooting for antigen retrieval methods * Antibody testing for determination of optimal conditions * Immunofluorescence of cultured cells * Tissue preparation for electron microscopy * Tissue and cell preparation for confocal microscopy * Antibody purification for use in immunohistochemistry * TUNEL staining in fixed sections for apoptosis studies Dual color TUNEL and PCNA or Ki-67 staining for apoptosis/proliferation index * In situ hybridization for localizing mRNA in tissue sections (fluorescent and colorometric visualization) Image analysis. The Core provided services to assist investigators with image acquisition and documentation of results using our image analysis workstation. Using the Nikon Eclipse E400 series microscope, single images are captured, processed and printed with a fully functional computer workstation. The Image-Pro Plus software and NIH Image J are used for quantitative morphometric analyses. Serve as a training center. The Core has trained investigators, pre- and post-doctoral students as well as technicians of U54 investigators in the art and science of immunohistochemistry, in situ hybridization, image analysis and data processing.