Structural Biology of sulfotransferase and glycosyltrans

用于解毒和分泌的胞内STS硫酸盐外来化学物质，以及用于灭活和/或储存的硫酸盐生物信号分子，如类固醇激素和生物胺。某些硫酸盐化产物会致癌。高尔基膜STS对多糖硫酸盐的不同部位表现出高度的特异性。各种糖基转移酶合成多糖，为STS提供底物。我们的研究目标是阐明这些酶的结构和功能。为此，我们利用细菌表达系统生产了大量的重组蛋白，并对它们进行了结晶，解决了它们的三维结构。并用等温滴定卡计研究了溶液中各种分子间的相互作用。

Cytosolic STs sulfate xenochemicals for detoxification and secretion and also sulfate biological signal molecules such as steroid hormones and bioamines for inactivation and/or storage. Certain sulfated products become carcinogenic. Golgi-membrane STs display high specificity to sulfate distinct sites of polysaccharides. Various glycosyltransferases synthesize polysaccharides and provide STs with substrates. Our research objectives are to eluciadte structure and function of these enzymes. For this, we have used bacterial expression system to produce a large amount of recombinant proteins, have crystallized them, and have solved their 3-dimensional structures. Also Isothermal titration carlimetry is used to investigate various molecular interactactions in solution.