AB 3730XL, 96 CAP DNA ANALYZER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：基因分型的需求正在迅速增长，这是由于通过大量的关联研究对复杂性状的研究，以及大量的样本和SNP。与此同时，至少在目前的水平上，对微卫星基因分型和测序的需求仍然存在。因此，必须提高快速、廉价、高通量测序和基因分型的能力。最近实验室仪器的迅速增长很好地跟上了这种日益增长的需求。然而，这种高科技伴随着高昂的价格。事实证明，除了规模最大、资金最雄厚的实验室外，任何实验室都不可能找到资源，随着研究的进展从一种尖端技术转移到另一种尖端技术。为了解决这个问题，加州大学洛杉矶分校承诺通过提供最先进的核心设施系统来支持和促进所有科学家的研究。这些核心配备了人员和装备，使科学家能够将更多的时间集中在解决智力问题上，而不是更少地用于获取和学习新技术。加州大学洛杉矶分校测序和基因分型核心满足了当地科学界大量用户的研究需求。我们已经发现，未来需要一种廉价的方法来产生越来越多的SNP基因型。为了满足这一需要，同时继续支持我们所服务的科学家的测序和微卫星要求，核心需要更新其能力。我们正在为AB 3730XL，96毛细DNA分析仪申请资金。五年多来，Core已经成功地运行了这项技术的较旧型号。目前有两台AB3700型毛细管仪和一台AB3730S型、48型毛细管仪。这三台仪器目前正在接近满负荷运行，每天都在生成DNA序列、微卫星和SNPs。这3700台仪器已经连续运行了四年，很快就需要更换。3700不能运行SNP基因型，其产生的序列阅读长度较短，质量低于3730。与3730相比，3700的效率较低，更容易出现故障，而且运行成本和每个样本的成本都更高。该模式已停产，在所要求的资助期后两年内不会得到支持。因此，有必要用所要求的3730仪器替换3700。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The demand for genotyping is increasing rapidly, fuelled by research into complex traits through large association studies, with large numbers of samples and SNPs. At the same time, the need for microsatellite genotyping and sequencing continues to exist in at least their current levels. Therefore, the capacity for rapid, cheap, high-throughput sequencing and genotyping must increase. The recent rapid explosion in laboratory instrumentation is well able to keep pace with this increasing need. However, this high technology comes with a high price tag. It can prove impossible for any but the largest, most well-funded laboratories to find the resources to move from one cutting-edge technology to the next as their research progresses. To address this problem, the University of California, Los Angeles has made a commitment to supporting and facilitating the research of all its scientists by providing a system of state-of-the-art Core facilities. These Cores are staffed and equipped to allow scientists to concentrate more time on intellectual problem solving, and less on acquiring and learning new technologies. The UCLA Sequencing and Genotyping Core serves the research needs of a large user base in the local scientific community. We have identified a coming need for an inexpensive method to generate increasingly large numbers of SNP genotypes. In order to meet this need, while at the same time continuing to support the sequencing and microsatellite requirements of the scientists we serve, the Core needs to update its capacity. We are requesting funds for an AB 3730XL, 96 Capillary DNA Analyzer. The Core has been successfully operating older models of this technology for over five years. Currently there are two AB 3700 capillary instruments, and one AB 3730S, 48 capillary instrument. These three instruments are currently running close to capacity, generating DNA sequence, microsatellites, and SNPs on a daily basis. The 3700 instruments have been in continuous operation for four years, and will need to be replaced very soon. The 3700 cannot run SNP genotypes, and the sequence it produces has shorter read lengths and inferior quality to the 3730. The 3700 is less efficient, more prone to break-down, and has higher running costs and per-sample costs than the 3730. The model has been discontinued, and will not be supported within two years of the requested funding period. Therefore, it is necessary to replace the 3700s with the requested 3730 instrument.