Olympus FV 1000 Confocal Microscope for Neurobiology and Cellular Biology Researc

描述（由申请人提供）：本提案要求提供资金，用于购买Olympus Fluoview（FV）1000光谱单元激光扫描共聚焦显微镜，供NIH资助的研究者互动小组使用，主要位于赖特州立大学解剖学和生理学系。该单元还可与分布在医学院其他几个系和科学与数学学院的其他研究人员共享使用。这种新的共焦系统将服务于两个主要目的，1）更换我们六年的奥林巴斯FX和2）升级我们的共焦仪器的能力。新系统提供的主要进步包括：a）超出我们当前两个通道限制的多种荧光染料标记; B）激光照明的最佳控制; c）改进的荧光染料分离，即使当发射曲线密切相关时也是如此; d）改进的文件处理、保存和导出，以便在2D和3D空间中进行进一步的定量分析。该仪器将主要由五名研究人员组成的核心小组使用，他们将组成一个合作单位，在发育，损伤和损伤恢复期间修改突触和电路的共同项目和共同科学兴趣。他们还在成像设备和定量方法方面积累了广泛的专业知识，其中许多都需要奥林巴斯FV 1000系统的改进功能。其他用户的研究主题各不相同，从神经元的体积或pH控制到红细胞和神经元的细胞钙转运机制和氯稳态，再到呼吸功能的中枢神经系统控制。大多数研究人员（主要或次要用户）都是通过NIH资助的。为了促进维护和公平使用，该仪器将被整合到大脑研究中心的显微镜和成像设施中。该核心设施成立于2000年，其使命是为主要（但不限于）位于医学院的研究人员提供成像和组织学需求（电子显微镜，图像分析，神经元追踪，共聚焦显微镜，切片术和超微切片术），并强调神经科学研究。因此，收购奥林巴斯FV 1000系统将大大加强核心研究人员的研究计划，但它也将对赖特州立大学更广泛的研究社区产生有益的影响。

DESCRIPTION (provided by applicant): This proposal requests funds to purchase an Olympus Fluoview (FV) 1000 Spectral Unit Laser Scanning Confocal Microscope to be utilized by an interactive group of NIH-funded investigators, primarily located in the Department of Anatomy and Physiology at Wright State University. The unit will be also available for shared use with other investigators distributed throughout several other Departments of the Medical School and the College of Science and Mathematics. This new confocal system will serve two major purposes, 1) replacement of our six year-old Olympus FX and 2) upgrade our confocal instrumentation capabilities. Major advances provided by the new system include a) multiple fluorochrome labeling beyond our current two channel limitations; b) optimal controls of laser illumination; c) improved fluorochromes separation, even when emission curves are closely related and d) improved file handling, save and export for further quantitative analyses in 2D and 3D space. The instrument will be mostly used by a core group of five investigators that will form a collaborative unit with shared projects and common scientific interests on the modification of synapses and circuits during development, injury and recovery from injury. They have also accumulated a broad range of expertise with imaging equipment and quantitative methods, many of which require the improved capabilities of the Olympus FV 1000 system. The research topics from further users are varied and range from volume or pH control in neurons to cellular calcium transport mechanisms and chloride homeostasis in red blood cells and neurons to central nervous system control of respiratory function. The majority of investigators (major or minor users) are funded through NIH. To promote maintenance and equitable use, the instrument will be integrated within the microscopy and imaging facilities of the Center for Brain Research. This core facility was established in 2000 with the mission of providing imaging and histological needs (electron microscopy, image analysis, neuron tracing, confocal microscopy, microtomy and ultramicrotomy) to researchers located primarily (but not exclusively) in the School of Medicine and with an emphasis in Neuroscience research. As such, the acquisition of the Olympus FV 1000 systems will significantly enhance the research programs of the core investigators, but it will also have a beneficial impact on the broader research community at Wright State University.