FUNDS TO ACQUIRE A JEOL JSM-7401F FESEM: INFECTIOUS DISEASE

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：本建议书要求为JEOL JSM-7401F场发射扫描电子显微镜提供资金，该显微镜具有嵌入式数字成像、光束减速技术和高分辨率背散射电子成像。爱荷华大学有一台扫描电子显微镜(1989年收购的日立S-4000型)为整个校园服务。目前的系统无法在低加速电压下提供免疫金标记样本的图像，因此迫切需要第二个系统，因为只有一个系统严重限制了研究人员的访问。2004年2月1日至2005年1月31日，来自22个系、6所高校54个教职工实验室的87名调查人员使用了S-4000型扫描电子显微镜。在54个教员实验室中，有30个NIH每年资助的研究补助金总额超过3800万美元。为了更好地支持这项研究，准备好访问最先进的仪器是至关重要的。大量使用需要调查人员提前2-3周预订日立S-4000型扫描电子显微镜。该系统一天24小时可供调查人员使用。此外，平均每学期有20名学生注册参加正式的显微镜研究课程。此外，我们亦定期举办结构设计短期课程和工作坊。S-4000的维修停机时间已经接近25%，这是由于非常频繁的使用、难以获得更换部件和缺乏经验的服务支持。新的FESEM将在爱荷华大学中央显微镜研究设施(CMRF)的监督下，方便地安装在埃克斯坦医学研究大楼的80室。管理、维护以及高级技术援助和培训将由经验丰富的工作人员提供。机构承诺很强，连续为一名研究助理提供39,000.00美元的工资支持，为年度服务合同提供14,000.00美元，以及12,550.00美元用于升级JEOL JSM-7401F上使用的低温实验台。提供研究描述的五名由NIH资助的教职员工是S-4000扫描电子显微镜的最大用户。Apicella将研究人类致病菌。威尔士将研究与Bardet-Biedl综合征和其他疾病有关的人类呼吸道上皮细胞生物学。格罗斯参与了人类疱疹病毒感染机制的确定。威尔逊检查了利斯曼病受损的人类巨噬细胞。斯塔纳将研究囊性纤维化患者中嗜血杆菌生物膜与感染和炎症的关系。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests funds for a JEOL JSM-7401F Field Emission Scanning Electron Microscope with embedded digital imaging, beam decelerating technology and high resolution backscattered electron imaging. The University of Iowa has one SEM (a Hitachi S-4000 acquired in 1989) to serve the entire campus. The current system cannot provide images of immunogold labeled samples at low accelerating voltages and a second system is critically needed because having only one system is severely limiting access by investigators. From February 1, 2004 to January 31, 2005, 87 investigators from 54 faculty labs in 22 departments and 6 colleges used the S-4000 SEM. 30 of the 54 faculty labs have NIH funded research grants totaling over $38,000,000.00 annually. To better support this research, it is critical to have ready access to state-of-the-art instrumentation. Heavy usage requires Investigators to reserve the Hitachi S-4000 SEM 2-3 weeks in advance. The system is made available 24 hrs a day to accommodate investigators. In addition, an average of 20 students a semester enroll in formal Microscopy Research classes. SEM short courses and workshops are also offered on a regular basis. Downtime for maintenance on the S-4000 has been nearly 25% and is due to the combination of very heavy use, difficulty in obtaining replacement parts and inexperienced service support. The new FESEM would be conveniently located in room 80 of the Eckstein Medical Research Building under the supervision of The University of Iowa Central Microscopy Research Facility (CMRF). Administration, maintenance as well as high-level technical assistance and training will be provided by experienced staff. Institutional commitment is strong, providing $39,000.00 in salary support for a Research Assistant and $14,000.00 towards the annual service contract on a continuing basis as well as $12,550.00 to upgrade a cryostage to be used on the JEOL JSM-7401 F. The five NIH funded faculty providing research descriptions are the heaviest users of the S-4000 SEM. Apicella will study human pathogenic bacteria. Welsh will investigate human airway epithelia cell biology in relation to Bardet- Biedl syndrome and other diseases. Grose is involved in determining the mechanism of human herpes virus infections. Wilson examines Leismania compromised human macrophages. Starner will study the relationship of Haemophilus biofilms to infection and inflammation in Cystic Fibrosis patients.