WHOLE ANIMAL IMAGING TO COMPARE TRANSGENE EXPRESSION IN RODENTS AND PRIMATES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。目的：在过去的十年里，已经发展了各种技术，可以对活体动物中表达的报告基因进行非侵入性成像。核磁共振、正电子发射计算机断层成像和光学成像技术都已经发展起来，而且每种技术都有其优缺点。光学技术之所以成为可能，是因为灵敏而廉价的ccd相机的发展，以及意识到人体内部发射的光在令人惊讶的极少关注的情况下被传输到外部。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: In the last decade, a variety of techniques have been developed which allow noninvasive imaging of reporter genes expressed within a live animal. NMR, PET and optical imaging techniques have all been developed and each has its advantages and disadvantages. The optical techniques were made possible by the development of sensitive and inexpensive CCD cameras and also by the realization that light emitted in the interior of the body is transported to the exterior with suprisingly little attentuation.