TECHNOLOGY FOR COMPREHENSIVE ANALYSIS OF GLUCONEOGENESIS IN PATIENTS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。总体目标是利用氘和碳13的NMR透明度，加上高信息产量，开发新的工具来探测动物模型和人类受试者的代谢。在离体肝脏中的验证研究、辅助实验设计的数学模拟、优化灵敏度的改进探针设计、自动光谱分析和探测特定途径的新示踪剂标记模式都在开发中。自动化溶液相合成仪现在通常用于处理血浆样品。Core继续支持Core 3中描述的人类应用程序，以及与众多协作者的交互

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal is to take advantage of the NMR transparency of deuterium and carbon 13, plus the high information yield to develop new tools to probe metabolism in animal models and in human subjects. Validation studies in isolated livers, mathematical simulations to assist in experimental design, improved probe design to optimize sensitivity, automated spectral analysis and new tracer labeling patterns to probe specific pathways are all under development. The automated solution phase synthesizer is now routinely used for processing of plasma samples. The Core continues to support applications to humans as described in Core 3, plus interactions with numerous collaborator