EFFECTS OF SREBP 1C OVEREXPRESSION IN FAT ON LIVER METABOLISM

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。基于核磁共振（NMR）同位素分析的肝通量谱分析（LFP）已应用于小鼠、大鼠和人类，但该技术对肝脏生理学受控变化的敏感性知之甚少。两个子项目正在进行中。首先，aP2-SREBP-1c转基因小鼠在脂肪组织中过表达甾醇调节元件结合蛋白1c （SREBP-1c）的截断核版本。这种蛋白的过度表达导致参与脂肪酸合成的酶的升糖。其次，我们正在研究氧化还原状态对肝葡萄糖生成途径的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Liver flux profiling (LFP), based upon nuclear magnetic resonance (NMR) isotopomer analysis, has been applied in mice, rats and humans yet little is known about the sensitivity of this technique to controlled changes in hepatic physiology. Two subprojects are underway. First, the aP2-SREBP-1c transgenic mouse over expresses a truncated nuclear version of the sterol regulatory element binding protein-1c (SREBP-1c) in adipose tissue. Overexpression of this protein results in the upregluation of the enzymes involved in fatty acid synthesis. Second, we are examining the effects of redox state on pathways of hepatic glucose production.