MEASURING TWO-PHOTON CROSS SECTIONS WITH PCH

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们正在尝试用光子计数直方图（PCH）测量双光子吸收截面。PCH分析可以告诉我们荧光样品的分子亮度和平均粒子数。利用分子亮度和量子产率，我们可以计算出相对于已知标准荧光素的横截面。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are trying to measure two-photon absorption cross sections using the photon counting histogram (PCH). PCH analysis can tell us the molecular brightness and average particle number of our fluorescent sample. Using the molecular brightness and quantum yield we can then calculate the cross section relative to a known standard, fluorescein.