MR MICROSCOPY OF DEVELOPING RAT EMBRYO

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。该项目的全球目标是开发大鼠胚胎自动形态学分析的策略。将从妊娠Sprague道利大鼠中采集标本。然后根据先前研究中使用的染色方法对其进行固定。根据样本的大小，将12天、13天、14天、15天、16天、17天和18天的胎仔分别浸入Bouins/Prohance溶液（20/1）中5分钟、10分钟、20分钟、30分钟、1小时、1.5小时和3.5小时，以实现固定。扫描方法将包括使用适合胚胎大小的适当线圈，以尽可能好地配合。将使用不同序列采集3D图像：T1加权、T2加权和弥散加权，信噪比较高。这些不同加权图像的组合将用于开发大鼠胚胎的自动分割。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The global goal of this project is to develop strategies for automatic morphology analysis in the rat embryo. The specimens will be harvested from pregnant Sprague Dawley rats. They will be then fixed accordingly to the staining method used in the previous study. Depending on the size of the specimen, the fixation will be achieved by immersion in a Bouins/Prohance solution at 20/1 for 5 min, 10 min, 20 min, 30 min, 1 hr, 1.5 hrs, and 3.5 hrs for respectively 12-day, 13-day, 14-day, 15-day, 16-day, 17-day, and 18-day fetuses. The scanning method will include the use of an appropriate coil adapted to the size of the embryo to fit as well as possible. 3D images will be acquired using different sequences: T1-weighted, T2-weighted, and Diffusion-weighted, at high Signal-to-noise ratios. A combination of these different weighted images will be used to develop an automated segmentation of the rat embryo.