MULTIMODAL SPECTROSCOPY PROBE DEVELOPMENT

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们正在研究结合来自反射和荧光的诊断信息和通过拉曼光谱获得的诊断信息来诊断动脉粥样硬化的实用性。因此，正在开发一种单一的光谱探测器，以用于所有三种模式。组合的探测器将从现有的临床FastEEM和拉曼光源获取激发光，从单一源点传输和收集来自组织的光。组合探头的设计能够传输和收集300-1000 nm光谱范围内的光。基于已在手术室成功使用的在体拉曼探头，组合探头将利用单一的锥形激发光纤耦合不同的光源，将激发和发射滤光片与专门设计的光学涂层结合在一起，以抑制光纤背景，并实现所需信号的有效提取。采集的纤维将被分割，并被引导到两个临床系统进行光谱分析和参数提取。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the utility of combining diagnostic information from reflectance and fluorescence to that obtained by Raman spectroscopy to diagnose atherosclerosis. As a result, a single spectral probe is being developed to be used with all three modalities. The combined probe would take excitation light from existing clinical FastEEM and Raman sources, deliver and collect the light from the tissue from a single source point. The combined probe is designed to be capable of delivering and collecting light in the 300-1000 nm spectral range. Based on the in vivo Raman probe that has been successful used in the surgical theatre, the combined probe will utilize a single tapered excitation fiber to couple the different light sources, incorporate excitation and emission filters with specially designed optical coatings to suppress fiber background and enable efficient extraction of the desired signal. Collection fibers would be split up and be directed to the two clinical systems for spectral analysis and parameter extraction.