DETECTION OF VULNERABLE PLAQUES USING RAMAN, FLUORESCENCE AND REFLECTANCE SPECT

使用拉曼、荧光和反射光谱检测易损斑块

• 批准号: 7357953
• 负责人: OBRAD SCEPANOVIC
• 金额: $ 2.03万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357953
• 关键词: Raman spectrometry; carotid artery; cholesterol; collagen; diagnosis; fluorescence; fluorescence spectrometry; hemoglobin; hemorrhage; human; macrophage; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Vulnerable plaques are characterized by morphological features such as a thin or ulcerated fibrous cap, a large necrotic core, superficial foam cells, and intraplaque hemorrhage. We argue for the use of a multi-modal spectroscopic method, comprising Raman, diffuse reflectance, and intrinsic fluorescence spectroscopy, to detect these key morphological markers of vulnerable plaque. A preliminary study was performed in vitro on 17 human carotid artery specimens. Following the acquisition of the spectra, each specimen was histologically evaluated. It was determined that parameters obtained from diffuse reflectance spectroscopy, the hemoglobin concentration and the scattering parameter, can potentially be used to detect intraplaque hemorrhage and foam cells, respectively. A parameter extracted from the intrinsic fluorescence that relates to the amount of collagen in the topmost layers of the tissue can be used to sense the presence of a thin fibrous cap. Finally, a Raman spectroscopy parameter related to the amount of cholesterol and necrotic material can potentially be used to detect a necrotic core. The multi-modal spectroscopy method shows great promise to provide depth-sensitive and complementary morphological information about plaque composition. A larger study is needed to further validate the potential for diagnosis of vulnerable plaque.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。易损斑块的特征在于形态特征，例如薄的或溃疡的纤维帽、大的坏死核心、表面泡沫细胞和斑块内出血。我们主张使用多模态光谱方法，包括拉曼光谱、漫反射光谱和内在荧光光谱，来检测易损斑块的这些关键形态标记物。对 17 份人类颈动脉标本进行了体外初步研究。获取光谱后，对每个样本进行组织学评估。确定从漫反射光谱获得的参数、血红蛋白浓度和散射参数可分别用于检测斑块内出血和泡沫细胞。从内在荧光中提取的与组织最上层胶原蛋白数量相关的参数可用于感测薄纤维帽的存在。最后，与胆固醇和坏死物质的量相关的拉曼光谱参数可潜在地用于检测坏死核心。多模态光谱方法在提供有关斑块组成的深度敏感和补充形态信息方面显示出巨大的前景。需要进行更大规模的研究来进一步验证诊断易损斑块的潜力。