OPTICAL FIBER PROBE FOR BIOMEDICAL RAMAN SPECTROSCOPY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。体外实验表明，拉曼光谱技术能够诊断多种疾病。最近使用光纤探头进行的活体研究很有希望，但通常仅限于容易接触到的器官，通常需要相对较长的收集时间。我们已经实施了一种光学设计策略，通过表征来自组织的拉曼分布来充分利用系统吞吐量。这种方案优化了采集效率，最大限度地减少了噪声，并在1 S获得了能够收集高质量数据的小直径、高效率的拉曼探测器。通过对组织模型和几种体外组织类型的模拟和实验来测试性能，表明这种新的设计可以促进拉曼光谱作为临床实用技术的应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In vitro experiments have demonstrated the ability of Raman spectroscopy to diagnose a wide variety of diseases. Recent in vivo investigations performed with optical fiber probes were promising but generally limited to easily accessible organs, often requiring relatively long collection times. We have implemented an optical design strategy to utilize system throughput fully by characterizing the Raman distribution from tissue. This scheme optimizes collection efficiency, minimizes noise, and has resulted in small-diameter, highly efficient Raman probes that are capable of collecting high-quality data in 1 s. Performance has been tested through simulations and experiments with tissue models and several in vitro tissue types, demonstrating that this new design can advance Raman spectroscopy as a clinically practical technique.