DEVELOPMENT OF RAMAN SPECTROSCOPY FOR IN VIVO DIAGNOSIS OF ATHEROSCLEROSIS

我们正在开发用于活体诊断的拉曼光谱 动脉硬化。一种临床可用的诊断系统，其包括 830 nm全息透射式半导体激光器 带有F#2采集光学元件、陷波滤光片的光栅式光谱仪 对于瑞利散射光的抑制，一种AR涂层的深度耗尽 用于高灵敏度检测的(CCD)探测器已经组装完成。纤维 光学探头(在远端具有集成的介电滤光片 减少荧光/拉曼背景信号)用于导管 基于体内的研究目前正在进行一种设计、改进 和测试过程，以允许临床可接受的数据采集。 一旦该系统的性能得到评估，临床研究将 从克利夫兰临床基金会，克利夫兰，大都会医疗中心开始， 波士顿和荷兰莱顿大学医院。详细 对各种疾病状态及其进展的研究将 最终考虑到最佳的预防措施和评估 治疗方案疗效。

We are developing Raman spectroscopy for in vivo diagnosis of atherosclerosis. A clinically usable diagnostic system which includes a diode laser operating at 830 nm, holographic tranmissive grating-based spectrograph with F#2 collection optics, a notch filter for rejection of Rayleigh scattered light, an AR coated deep depletion (CCD) detector for high sensitive detection has been assembled. Fiber optic probes (with integrated dielectric filters at the distal tip to reduce fluorescence/Raman background signal) to be used in catheter based in vivo studies are currently undergoing a design, refinement and testing process to allow clinically acceptable data acquisition. Once the performance of the system is evaluated clinical studies will begin at the Cleveland Clin Fdn, Cleveland, MetroWest Medical Center, Boston, and Leiden University Hospital, The Netherlands. Detailed studies of various disease states and their progression will eventually allow for optimal preventative measures and assessment of treatment protocol efficacy.