PROPERTIES OF SINGLE ASSEMBLIES OF LIGHT HARVESTER LH2

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们正在研究LH2配合物和可以从它们可逆提取的上二肽的单组装中的光漂白和转移动力学。每个LH2包含18个细菌叶绿素（B850）和9个收集器（B800）。研究了不同条件下的荧光寿命、极化、相关次数和漂白动力学。主要目标包括测量长时间尺度的蛋白质动力学。先前的极化实验已经揭示了单体LH2组装中与激子迁移相关的构象动力学。此外，来自高激发态的蓝色辐射最近被检测和研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are examining the photobleaching and transfer dynamics in single assemblies of the LH2 complexes and the up-dipeptides that can be reversibly extracted from them. Each LH2 contains eighteen bacteriochlorophylls (B850) and nine collectors (B800). The fluorescence lifetimes, polarization, correlation times and bleaching kinetics are being studied under a variety of conditions. The primary goals include measurements of long time scale protein dynamics. Previous polarization experiments have revealed conformational dynamics associated with excitonic migration in the monomeric LH2 assembly. Furthermore, blue emission originating from higher excited states was recently detected and investigated.