RAMAN FLOW CYTOMETRY FOR DRUG DISCOVERY AND DIAGNOSTICS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。对生物系统进行定量、高通量分子测量的能力是许多生物医学研究领域的关键需求。生物工程研究伙伴关系(BRP)旨在开发一个强大的新分析平台，用于基于拉曼流式细胞术的高通量筛选和选择。这一合作伙伴关系将开发新的分析仪器、用于化学合成和筛选的光学编码聚合物树脂，以及用于敏感报告和编码的具有独特光学性能的纳米结构材料。这项新技术将对流动中的单个颗粒进行拉曼光谱，以实现在敏感的多路检测、药物发现和诊断方面的新应用。拉曼流式细胞仪仪器和应用将由来自学术界、政府和工业界的工程师、生物学家和化学家组成的合作伙伴开发。在合作的第一年，我们将改装一种商用粒子分选器，从单个粒子中检测单个拉曼振动带，并根据这些粒子的光学特征对这些粒子进行分类。在2-5年内，我们将发展收集和分析单个粒子的完整拉曼光谱的能力。同时，该伙伴关系将为多路分子分析和分离开发新的编码和报告策略。这项拉曼流式细胞术技术将应用于细菌病原体及其毒素的治疗和诊断的发展。拉曼流式细胞术将是一种重要和通用的新的分析和分离能力，除了这里提出的应用之外，它还将影响许多基础和应用生物医学研究领域。这一合作项目推动了核心研发项目2(分子组装)和4(光谱流式细胞术)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to make quantitative, high throughput molecular measurements of biological systems is a critical need for many areas of biomedical research. This Bioengineering Research Partnership (BRP) aims to develop a powerful new analytical platform for high throughput screening and selection based on Raman Flow Cytometry. This Partnership will develop new analytical instrumentation, optically encoded polymer resins for chemical synthesis and screening, and nanostructured materials with unique optically properties for sensitive reporting and encoding. The new technology will perform Raman spectroscopy on single particles in flow to enable new applications in sensitive multiplexed detection, drug discovery, and diagnostics. The Raman Flow Cytometry instrumentation and applications will be developed by a Partnership involving engineers, biologists, and chemists from academia, government and industry. In the first year of the Partnership, we will modify a commercial particle sorter to detect individual Raman vibrational bands from single particles and sort these particles based on their optical signature. In Years 2-5, we will develop the ability to collect and analyze complete Raman spectra from single particles. In parallel, the partnership will develop new encoding and reporting strategies for multiplexed molecular analysis and separation. This Raman Flow Cytometry technology will be applied to the development of therapeutics and diagnostics for bacterial pathogens and their toxins. Raman Flow Cytometry will be an important and general new analytical and separation capability that will impact many areas of basic and applied biomedical research in addition to the applications proposed here. This collaborative project drives Core R&D Projects 2 (Molecular Assembly) and 4 (Spectral Flow Cytometry).