CHAPERONIN (SULFOLOBUS SHIBATAE)

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该项目将重点研究来自超嗜热古菌Sulfolobus shibatae的II族伴侣蛋白。柴田的伴侣蛋白是在所有生长条件下细胞中最丰富的一些蛋白质。该项目的目标是使用低温电子显微镜来：1)研究伴侣蛋白双环，称为“玫瑰花体”，是否在体内具有功能形式，或者是否存在一些更高阶的结构；2)表征伴侣蛋白的高阶结构，阐明它们如何在体内发挥作用，并研究它们如何用于纳米技术；3)观察热休克前后细胞中的伴侣蛋白结构。除了研究伴侣蛋白在体内的结构和位置外，我们还将研究与脂质体和脂质层相关的伴侣蛋白结构。这将支持我们在体内寻找这些伴侣蛋白，并将提供关于伴侣蛋白高阶结构的重要结构信息，我们已经将其用于纳米技术的应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project will focus on group II chaperonins from the hyperthermophilic archaeon, Sulfolobus shibatae. The chaperonins in S. shibatae are composed of some of the most abundant proteins in the cells under all growth conditions. The goals of this project are to use cryo-electron microscopy to: 1)investigate if the chaperonin double rings, called "rosettasomes", are the functional form in vivo or if some higher-order structures are present; 2) characterize the higher-order structures of chaperonins to elucidate how they may function in vivo and investigate how they can be used in nanotechnology; and 3) visualize the chaperonin structures in cells before and after heat shock. In addition to investigating the structure and location of chaperonins in vivo, we will investigate chaperonin structures in association with liposomes and lipid layers. This will support our search for these chaperonins in vivo and will provide important structural information about higher-order structures of chaperonins that we have been using for applications in nanotechnology.