SAN1 INTERACTIONS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。酵母蛋白San1是一种泛素蛋白连接酶，在泛素介导的胞核异常蛋白的蛋白酶体降解中发挥作用。目前尚不清楚San1是单独发挥作用，还是有对其功能至关重要的同类互动伙伴。我们将使用酵母双杂交方法寻找与San1相互作用的蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The yeast protein San1 is a ubiquitin-protein ligase that functions in the ubiquitin-mediated proteasomal degradation of aberrant proteins in the nucleus. It is not clear if San1 functions alone, or has cognate interacting partners vital to its function. We will search for San1-interacting proteins using the yeast 2-hybrid method.