PROTEOMIC PROFILING OF YEAST UBIQUITINOME UNDER DIFFERENT CONDITIONS

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In recent years, global genome analyses have identified the transcriptional changes that occur in cells after exposure to various growth and environmental conditions. Although there is much regulation at the level of transcription, there is also a significant amount of regulation at the post-translational level. One of the most broadly used post-translational regulatory mechanisms is the ubiquitination of proteins to control their stability, localization, activity, and/or interactions. To gain a deeper sense of how the cell globally regulates its protein complement, we are undertaking a series of large scale proteomic efforts to identify the global changes in protein ubiquitination as cells experience different growth or environmental conditions, including exposure to DNA, RNA and protein damaging agents. Yeast is an excellent model organism to pursue such studies because it is both economical and tractable.