PROTEOMIC PROFILING OF YEAST UBIQUITINOME UNDER DIFFERENT CONDITIONS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 近年来，全球基因组分析已经确定了细胞在各种生长和环境条件下发生的转录变化。虽然在转录水平上有很大的调控，但在翻译后水平上也有相当数量的调控。最广泛使用的翻译后调控机制之一是蛋白质的泛素化，以控制其稳定性、定位、活性和/或相互作用。为了更深入地了解细胞如何在全球范围内调节其蛋白质补体，我们正在进行一系列大规模的蛋白质组学努力，以确定随着细胞经历不同的生长或环境条件，包括暴露在DNA、RNA和蛋白质损伤剂中，蛋白质泛素化的全球变化。酵母是进行这类研究的一个很好的模式生物，因为它既经济又容易驯化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In recent years, global genome analyses have identified the transcriptional changes that occur in cells after exposure to various growth and environmental conditions. Although there is much regulation at the level of transcription, there is also a significant amount of regulation at the post-translational level. One of the most broadly used post-translational regulatory mechanisms is the ubiquitination of proteins to control their stability, localization, activity, and/or interactions. To gain a deeper sense of how the cell globally regulates its protein complement, we are undertaking a series of large scale proteomic efforts to identify the global changes in protein ubiquitination as cells experience different growth or environmental conditions, including exposure to DNA, RNA and protein damaging agents. Yeast is an excellent model organism to pursue such studies because it is both economical and tractable.