PROTEOMIC PROFILING OF YEAST UBIQUITINOME UNDER DIFFERENT CONDITIONS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 近年来，全球基因组分析已经确定了暴露于各种生长和环境条件后细胞中发生的转录变化。 虽然在转录水平有很多调节，但在翻译后水平也有大量调节。 最广泛使用的翻译后调节机制之一是蛋白质的泛素化以控制其稳定性、定位、活性和/或相互作用。 为了更深入地了解细胞如何全面调节其蛋白质补体，我们正在进行一系列大规模的蛋白质组学研究，以确定细胞经历不同生长或环境条件（包括暴露于DNA，RNA和蛋白质破坏剂）时蛋白质泛素化的全球变化。 酵母是一个很好的模式生物进行这样的研究，因为它是既经济又易于处理。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In recent years, global genome analyses have identified the transcriptional changes that occur in cells after exposure to various growth and environmental conditions. Although there is much regulation at the level of transcription, there is also a significant amount of regulation at the post-translational level. One of the most broadly used post-translational regulatory mechanisms is the ubiquitination of proteins to control their stability, localization, activity, and/or interactions. To gain a deeper sense of how the cell globally regulates its protein complement, we are undertaking a series of large scale proteomic efforts to identify the global changes in protein ubiquitination as cells experience different growth or environmental conditions, including exposure to DNA, RNA and protein damaging agents. Yeast is an excellent model organism to pursue such studies because it is both economical and tractable.