ANTIGEN INVOLVED IN ATYPICAL BINDING OF CHRONIC LYMPHOCYTIC LEUKEMIA CELLS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。慢性淋巴细胞白血病（CLL）的特点是造血器官中单克隆B淋巴细胞的积累。在少数病例中，CLL细胞聚集在单个非典型部位；这种不寻常分布的机制以前没有被研究过。来自严重前列腺浸润患者的CLL细胞被发现与培养的前列腺细胞结合，但不与结肠、乳腺或膀胱细胞结合。融合CLL细胞与小鼠-人异骨髓瘤细胞系，杂交瘤设计表达真正的CLL单克隆IgM，特异性结合前列腺细胞。该实验旨在表征与CLL IgM结合的抗原，该抗原被认为是介导这种非典型前列腺浸润的化合物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Chronic lymphocytic leukemia (CLL) is characterized by an accumulation of monoclonal B lymphocytes in the hematopoietic organs. In a few cases, CLL cells accumulate in a single atypical site; the mechanism for this unusual distribution has not been studied previously. CLL cells from patients with heavy prostate infiltration were found to bind to cultured prostate cells, but not to colon, breast, or bladder cells. Fusion CLL cells with a mouse-human heteromyeloma cell line, hybridomas designed to express the authentic CLL monoclonal IgM, bind specifically to prostate cells. The experiments are designed to characterize the antigen bound to the CLL IgM, which is indicated to be the compound mediating this atypical prostate infiltration.