SELENATE REDUCTASE: A MOLYBDENUM-CONTAINING ENZYME FROM THAUERA SELENATIS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。在某些生境中，特别是在含硒土壤的农业排水沃茨中，硒酸盐是一种丰富的含氧阴离子。能够将硒酸盐还原为亚硒酸盐和可溶性较低的元素硒的生物体作为生物修复的潜在介质是有意义的。其中一种生物是Thauera selenatis，它使用的是硒酸还原酶。它是一种周质酶，由100 kDa、37 kDa和23 kDa的三个异源亚基组成。每摩尔酶含有1摩尔钼、12摩尔铁和8摩尔酸不稳定硫。本项目旨在利用多波长反常色散（MAD）技术解决硒酸还原酶的结构问题。将收集关于铁吸收边的X射线衍射数据和用于确定相的异常差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Selenate is an abundant oxyanion in some habitats, particularly in agricultural drainage waters from seleniferous soils. Organisms capable of reducing selenate to selenite and the less soluble elemental selenium are of interest as potential mediators of bioremediation. One organism capable of this is Thauera selenatis, using the enzyme selenate reductase. It is a periplasmic enzyme, consisting of three heterologous subunits at 100 kDa, 37 kDa and 23 kDa. It contains 1 mole of molybdenum, 12 moles of iron and 8 moles of acid-labile sulfur per mole of enzyme. This project aims to solve the structure of selenate reductase by the technique of multiple wavelength anomalous dispersion (MAD). X-ray diffraction data will be collected about the iron absorption edge and the anomalous differences used to determine phases.