CRYSTAL STRUCTURES OF CLIC ION CHANNELS, SERPIN COMPLEXES, RNA BINDING PROTEINS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们的建议着眼于几种蛋白质系统的结构，在每种情况下，我们的目标是映射蛋白质中发生的功能重要的结构转变。CLIC 1是一类新的氯离子通道的成员。这些蛋白质以可溶性和完整膜形式存在。我们刚刚解出了CLIC 1的第一个结构，它是可溶形式的，我们有了第一个中间态的晶体，它正向膜结合形式过渡。Sm/Lsm蛋白形成真核细胞中许多RNP（包括剪接体、端粒酶和降解体）的基础。它们也存在于古细菌中。我们已经完成了一个古细菌LSM复合物的结构，最近与RNA形成了一个复合物。我们希望确定这种结构和更高级的配合物。PE 545是一种50 kDa的捕光蛋白，其以衍射至少为0.86的形式结晶，我们已经在1.1处解决了该结构，并旨在将其提高到最大分辨率。我们还有前折叠蛋白，伴侣蛋白和丝氨酸蛋白酶抑制剂的晶体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our proposal looks at the structures of several protein systems, in each case, we aim to map the functionally important structural transitions that occur in the protein. CLIC1 is a member of a new class of chloride ion channel. These proteins exist in both a soluble and integral membrane forms. We have just solved the first structure of CLIC1 in the soluble form and we have crystals of the first intermediate state in the transition to the membrane bound form. The Sm/Lsm proteins form the basis for many RNPs in eucaryotic cells (including the spliceosome, telomerase and degradosome). They are also present in archaea. We have completed the structure of an archaeal Lsm complex & have recently formed a complex with RNA. We wish to determine this structure and higher order complexes. PE545 is a 50 kDa light harvesting protein that crystallizes in a form that diffracts to at least 0.86 ¿¿ We have solved this structure at 1.1 ¿¿and aim to improve this to maximal resolution. We also have crystals of prefoldins, chaperonins and serpins.