CHRISTOPHER GARCIA PRT TIME

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我的实验室对与人类健康和疾病相关的细胞表面受体/配体相互作用的结构方面感兴趣。我们从事许多受体胞外结构域的表达和结晶研究，包括单独的和与其配体复合的。我们研究的受体系统主要在免疫和神经系统中，特别强调理解分子识别中的交叉反应性。我们的大多数项目都严重依赖于在同步加速器设施中收集X射线数据的能力，因为我们可以生长大型和异质性蛋白质复合物的晶体通常较小且质量适中。内部X射线源不足以测量我们大多数晶体的有用数据，这些晶体的数量通常很少，并且从我们用来制造重组蛋白的哺乳动物表达源生产非常劳动密集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. My laboratory is interested in structural aspects of cell-surface receptor/ligand interactions with relevance to human health and disease. We are engaged in expression and crystallization studies of numerous receptor extracellular domains both alone and in complex with their ligands. The receptor systems we study are primarily in the immune and nervous systems, with a special emphasis on understanding cross-reactivity in molecular recognition. Most of our projects are critically dependent on the ability to collect x-ray data at a synchrotron facility due to the generally small and moderate quality of the crystals which we can grow of large and heterogeneous protein complexes. In-house x-ray sources are not sufficient to measure useful data on most of our crystals, which are often few in number and extemely labor intensive to produce from the mammalian expression sources we utilize to make recombinant proteins.