STRUCTURE BASED APPROACH TO ISSUES IN ZINC FINGER RECOGNITION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。Aart是由斯克里普斯研究所Barbas实验室的大卫西格尔教授设计的一种六指锌指蛋白。它是晶体学研究的绝佳选择，因为它结合的几个核苷酸三联体是不寻常的，并且因为它可以提供与长度超过三个手指的锌指蛋白相关的菌株的洞察力。这种蛋白质以前在这个实验室里被结晶过。在APS BL 14-BMC收集了2.95的完整天然数据集（P1 a = 40.57 B = 69.09 c = 70.84 alpha = 101.18 beta = 94.36 gamma = 105.30），在不对称单元中有两个蛋白质分子和两个22碱基对的DNA双链体。同步辐射是高分辨率数据采集和结构解析的必要手段。计划使用这种晶体形式的溴化DNA衍生物进行多波长异常色散数据收集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aart is a six-finger zinc finger protein designed by Prof. David Segal in the Barbas lab at the Scripps Research Institute. It is an excellent choice for crystallographic studies because several of the nucleotide triads it binds are unusual and because it may offer insight into the strain associated with zinc finger proteins longer than three fingers in length. The protein has been crystallized in this lab previously. A complete native data set has been collected to 2.95 ¿¿at APS BL 14-BMC (P1 a = 40.57 ¿¿b = 69.09 ¿¿c = 70.84 ¿¿alpha = 101.18¿¿ beta = 94.36¿¿ gamma = 105.30¿¿) there are two protein molecules and two twenty-two base pair DNA duplexes in the asymmetric unit. Synchrotron radiation is necessary for high resolution data collection as well as structure solution. Multi wavelength anomalous dispersion data collection is planned using brominated DNA derivatives of this crystal form.