OPTICAL BIOSENSOR FOR THE EARLY DETECTION OF BREAST CANCER

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在第一阶段，我们建议确定在硝酸纤维过滤器中捕获的乳房分泌的蛋白质(特别是CEA)的测量是否可以用于检测乳腺癌。为此，我们将从需要乳房活检的患者的乳头分泌物中获取硝酸纤维素纸样本，以确定该检测的敏感性和特异性。在第二阶段，已经为检测CEA而优化的光学生物传感器将用于检测在血清和乳房分泌物中发现的针对CEA的荧光标记抗体。肿瘤标志物癌胚抗原(CEA)将作为乳腺癌的早期标志物在血液和乳头分泌物中作为模式系统。最终，生物传感器将是一种手持设备，可以用于在服务点环境中测试微量的血液或分泌物。这种设备的出现应该可以更早、更方便地检测CEA，并最终检测其他肿瘤标志物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the first stage we propose to determine if the measurement of proteins (specifically CEA) secreted by the breast captured in a nitrocellulose filter can be used in detecting breast cancer. For that purpose we will obtain samples from nipple secretions in nitrocellulose paper from patients requiring a breast biopsy to determine the sensitivity and specificity of the assay. In a second stage an optical biosensor that has been optimized for the detection of CEA will be used to detect fluorescent labeled antibodies directed against CEA found in the serum and breast secretions. The tumor marker carcinoembryonic antigen (CEA) will be used as an early marker for breast cancer in blood and nipple secretions as a model system. Ultimately, the biosensor will be a handheld device that can be used to test minute amounts of blood or secretions in a point of service context. The availability of such a device should allow earlier and more convenient testing for CEA and, eventually, for other tumor markers.