MLST scheme for the porcine pathogen Actinobacillus pleuropneumoniae

猪胸膜肺炎放线杆菌(APL)是引起世界范围内严重威胁生命的猪肺病的重要病原菌。细菌可以通过空气或直接接触从一头猪传到另一头猪。并不是所有的APL细菌都是相同的，已经开发出识别不同类型的方法(分型方案)。最广泛使用的一种方法，称为血清分型，可以识别10种不同类型的APL，主要基于APL可能具有的不同糖衣。了解APL的血清型有助于制造针对APL的疫苗，但对于当地和全国范围内存在的APL的个体形式，我们只能提供有限的信息。我们将开发一种名为MLST的分型方案，它使用APL特定基因的DNA序列。MLST的优势在于，它可以用于跟踪来自世界各地的单个APL表单。我们打算(1)将MLST应用于英国和欧洲的APL分离株(2)确定从猪身上分离的APL的血清型，以便对新旧方案进行比较，以及(3)确定在英国和欧洲使用哪种用于治疗受感染动物的抗生素是最好的。

Actinobacillus pleuropneumoniae (APL) is a bacterium that causes a world-wide economically important severe life threatening lung disease of pigs. Bacteria can be passed from pig to pig through the air or by direct contact. Not all APL bacteria are the same and methods to identify different types (typing schemes) have been developed. The most widely used one, called serotyping, recognises 10 different types of APL and is largely based on the different sugar coatings that APL may have. Knowing the serotype can help in making vaccines against APL but only gives us limited information as to the individual forms of APL that are around both locally and nationally. We are going to develop a typing scheme called MLST that uses the DNA sequence from specific genes of APL. MLST has the advantages that it can be used to track individual APL forms from all over the world. We intend to (1) apply MLST to UK and European isolates of APL (2) determine the serotype of APL isolated from pigs, to allow comparison of old and new schemes, and (3) determine which antibiotics, used to treat infected animals, are the best to use in the UK and Europe.