HIGH RESOLUTION STRUCTURE OF BACTERIOPHAGE P2

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。噬菌体、疱疹病毒和其他大型dsDNA病毒包含将DNA泵入预组装的前衣壳中的分子机器，引发成熟，伴随着一系列相关的颗粒大小变化、包装酶的释放、蛋白质结合以进行细胞结合以及激活颗粒以进行感染。这些事件与衣壳压力超过瓶装香槟10倍有关，并由一个蛋白质开关检测到，该开关将信号从颗粒内部传递到颗粒外部。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacteriophages, herpesviruses and other large dsDNA viruses contain molecular machines that pump DNA into preassembled procapsids, triggering maturation with an associated cascade of particle size change, release of packaging enzymes, binding of proteins for cell association and activation of the particles for infection. These events are associated with capsid pressures exceeding, by 10-fold, that of bottled champagne, and are detected by a protein switch that transduces a signal from inside to outside of the particle.