HIGH RESOLUTION STRUCTURE OF BACTERIOPHAGE P22

噬菌体 P22 的高分辨率结构

• 批准号: 8362443
• 负责人: John Emil Johnson
• 金额: $ 0.64万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362443
• 关键词: Bacteriophage P22; Bacteriophages; Binding Proteins; Capsid; Cells; DNA; Double Stranded DNA Virus; Enzymes; Event; Funding; Grant; Herpesviridae; Infection; Microscopy; Molecular; Molecular Machines; National Center for Research Resources; Particle Size; Principal Investigator; Proteins; Pump; Research; Research Infrastructure; Resolution; Resources; Signal Transduction; Source; Structure; United States National Institutes of Health; cost; particle; pressure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bacteriophages, herpesviruses and other large dsDNA viruses contain molecular machines that pump DNA into preassembled procapsids, triggering maturation with an associated cascade of particle size change, release of packaging enzymes, binding of proteins for cell association and activation of the particles for infection. These events are associated with capsid pressures exceeding, by 10-fold, that of bottled champagne, and are detected by a protein switch that transduces a signal from inside to outside of the particle.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 噬菌体、疱疹病毒和其他大型双链DNA病毒都含有分子机器 将DNA泵入预先组装好的前壳体，引发相关的 颗粒尺寸变化的级联，包装酶的释放，细胞蛋白质的结合 结合和激活用于感染的颗粒。 这些事件与 衣壳压力超过，10倍，瓶装香槟，并检测到一个 蛋白质开关，将信号从颗粒内部传递到外部。