Biomed Studies & Instrumentation in Proteomics/Glycomics

超出空间 但前提是。 该申请寻求支持蛋白质组学和糖组学领域的卓越中心和地区性、 在这一广泛的科学领域，国家和国际资源。蛋白质组学的研究与发展 印第安纳大学的设施目前得到了礼来公司一小部分赠款的支持 向印第安纳大学捐赠(印第安纳基因组计划)。重大装备和科学基础设施 在当地得到了支持，用于(A)蛋白质组学分析的仪器开发和(B)合作 与当地的生物医学界合作。此应用程序寻求对延续最新版本的支持 研究活动，并将这些活动扩大为国家和国际合作努力。这个 Pi和他的同事们提出了蛋白质组学和糖组学的新仪器和方法 几个领域(微型化分离技术及其与质谱学的耦合；微制造 蛋白质组学和糖组学设备；强大的质量分离技术的联用； 生物信息学)，并申请资金建造一台新的傅里叶变换ICR质谱仪。在新的 建议的技术，强调分离工具和质谱学的有效耦合 为高灵敏度的糖蛋白研究生成一个全面、优化的分析系统。双向 来自不同机构的生物医学科学家的合作努力包括一系列主题： 基础和临床癌症研究；内分泌学和新陈代谢研究；蛋白质组学 酒精中毒的糖生物学；细菌、酵母、植物和动物体内翻译后修饰蛋白的研究 果蝇。虽然印第安纳大学已经建立了一些合作关系，但美国的两个 大学和两个外国研究机构(在捷克共和国和瑞典)也将参加 交换学者、生物医学感兴趣的样本和科学知识。鉴于合作者 将由他们自己的赠款支持，要求为国际合作活动提供旅费。 在这笔赠款存在期间，蛋白质组/糖链服务活动的机制将得到发展。 (不受申请资金的支持)。蛋白质组学/糖组学领域的教育活动也将 开发，包括开发短期课程、研讨会方案和协调一致的 学生和博士后科学家。

EXCEED THE SPACE PROVIDED. This application seeks support for a center of excellence in proteomics and glycomics and a regional, national and international resource in this broad scientific area. The Proteomics Research and Development Facility at Indiana University has been currently supported by a small fraction of a grant from Lilly Endowment to Indiana University (Indiana Genomics Initiative). Major equipment and scientific infrastructure have been supported locally for (a) instrumental developments in proteomic analysis and (b) collaborations with the local biomedical community. This application seeks support for continuation of the most current research activities and enlargement of these activities to national and international collaborative efforts. The PI and his colleagues propose new instrumentation and methodologies for proteomics and glycomics in several areas (miniaturized separation techniques and their coupling to mass spectrometry; microfabricated devices for proteomics and glycomics; hyphenation of powerful mass separation technologies; bioinformatics) and request funding for building a new Fourier-transform ICR mass spectrometer. In the new proposed technologies, effective coupling of separation tools and mass spectrometry is emphasized to generate a total, optimized analytical system for high-sensitivity glycoprotein investigations. Two-way collaborative efforts involving biomedical scientists from various institutions include a range of topics: fundamental and clinically oriented cancer research; studies in endocrinology and metabolism; proteomics and glycobiology of alcoholism; studies of posttranslationally modified proteins in bacteria, yeast, plants and fruit flies. While a number of collaborations have already been established at Indiana University, two U.S. universities and two foreign research institutions (in Czech Republic and Sweden) will also participate in the exchange of scholars, biomedically interesting samples and scientific knowledge. Whereas the collaborators will be supported by their own grants, travel funds are requested for international collaborative activities. During the existence of this grant, the mechanism for proteomic/glycomic service activity will be developed (not supported by requested funds). Educational activities in the area of proteomics/glycomics will also be developed, including the development of short courses, a seminar program and concerted education of students and postdoctoral scientists.