Protein purification instrumentation in support of single molecule studies of genome integrity

支持基因组完整性单分子研究的蛋白质纯化仪器

• 批准号: 10386035
• 负责人: Eric C Greene
• 金额: $ 9.34万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10386035
• 关键词: Address; Biological; Cancer Biology; Cells; Chromosomal Rearrangement; Complex; DNA; DNA Double Strand Break; Development; Equipment; Event; Goals; Individual; Lead; Malignant Neoplasms; Methodology; Microscopy; Molecular; Nucleic Acids; Optics; Positioning Attribute; Proteins; Recombinant Proteins; Research; System; Technology; Time; Visualization; cancer genome; genome integrity; insight; instrumentation; interest; novel; prevent; protein purification; repaired; single molecule; success

My group has a long–standing interest in understanding the molecular mechanisms that contribute to cancer biology and genome integrity. To provide new insights into the mechanisms of genome integrity we have pioneered the development of DNA curtains, which allow for the direct visualization of hundreds to thousands of individual molecules in real–time by optical microscopy. This experimental platform is applicable to many types of protein–nucleic acid interactions and allows us to address questions about complex biological problems that cannot be addressed through traditional approaches. Our success in with this technology has uniquely positioned the lab to reveal new concepts regarding the mechanisms of genome integrity. Our use of this novel single molecule methodology is dependent upon access to highly purified recombinant proteins. This Supplemental Equipment request will allow us to replace our outdated protein purification system so that we can continue moving forward on our research efforts into understanding the mechanisms of genome integrity.

我的团队长期以来一直对了解分子机制感兴趣