Protein purification instrumentation in support of single molecule studies of genome integrity

我的团队长期以来一直对理解 有助于癌症生物学和基因组的完整性。提供新的见解的机制 为了保证基因组的完整性，我们率先开发了DNA窗帘， 通过光学实时直接可视化数百到数千个单个分子， 显微镜该实验平台适用于多种类型的蛋白质-核酸 相互作用，使我们能够解决复杂的生物学问题， 通过传统的方法来解决。我们在这项技术上的成功 定位实验室揭示新的概念，关于基因组完整性的机制。我们 这种新的单分子方法的使用依赖于获得高度纯化的 重组蛋白此补充设备请求将允许我们更换我们的 过时的蛋白质纯化系统，以便我们能够继续推进我们的研究 努力了解基因组完整性的机制。

My group has a long–standing interest in understanding the molecular mechanisms that contribute to cancer biology and genome integrity. To provide new insights into the mechanisms of genome integrity we have pioneered the development of DNA curtains, which allow for the direct visualization of hundreds to thousands of individual molecules in real–time by optical microscopy. This experimental platform is applicable to many types of protein–nucleic acid interactions and allows us to address questions about complex biological problems that cannot be addressed through traditional approaches. Our success in with this technology has uniquely positioned the lab to reveal new concepts regarding the mechanisms of genome integrity. Our use of this novel single molecule methodology is dependent upon access to highly purified recombinant proteins. This Supplemental Equipment request will allow us to replace our outdated protein purification system so that we can continue moving forward on our research efforts into understanding the mechanisms of genome integrity.