CRYSTAL STRUCTURE OF MEND

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。（1R,6R）-2-琥珀酰-6-羟基-2,4-环己二烯-1-羧酸（SHCHC）合成酶，也称为MenD，参与甲基萘醌（维生素K2）生物合成途径。该酶是ThDP依赖酶超家族的一部分，但它是已知的唯一一种催化ThDP加合物在不饱和羧酸盐的α -碳上的1,4加成的steter -like酶。这种酶的晶体结构对阐明这种重要酶的作用机理具有重要意义。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. (1R,6R)-2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase, also called MenD, participates in the menaquinone (Vitamin K2) biosynthetic pathway. The enzyme is a part of the superfamily of ThDP-dependent enzymes, however it is the only enzyme known to catalyze a Stetter-like 1,4-addition of a ThDP adduct to the alpha-carbon of an unsaturated carboxylate. The crystal structure of this enzyme will be important for clarifying the mechanism of this important enzyme.